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Microscopy Dictionary Terms

coverslip
A coverslip, also known as a cover glass or cover slip, is a thin and flat piece of transparent material typically made of glass or plastic. It is used in microscopy to cover a specimen mounted on a microscope slide. The primary purpose of a...
optical staining
With respect to microscopy, also called dispersive staining, optical staining the projection of colors through areas of a...
interphako interference microscopy
Measures the refractive indices axially from the fiber profile. Microscopy technique provides an interferogram with high...
dichroic mirror
A dichroic mirror, also known as a dichroic beamsplitter or interference filter beamsplitter, is an optical device that...
Golgi Staining Technique
The Golgi staining technique (also called the black reaction) makes it possible to look at the nervous system by rendering...
scanning coherent slope microscopy
Measures by heterodyning interferometry the local slope of a vibrating sample. The method allows the reconstruction of a...
metalens
A metalens, short for "metasurface lens," is a type of optical lens that uses nanostructured materials to manipulate light...
back-side illumination
Back-side illumination (BSI) is a technology used in imaging sensors, particularly in digital cameras, where the light is...
photoluminescence
Photoluminescence is a phenomenon in which a material absorbs photons (light) at one wavelength and then re-emits photons at...
acute bisectrix
The term acute bisectrix is used in mineralogy and crystallography to describe a specific optical property of minerals,...
Ti:sapphire laser
A Ti:sapphire laser is a type of solid-state laser that utilizes a titanium-doped sapphire crystal as the gain medium. The...
point-projection x-ray microscopy
A method of producing magnified images by x-rays. The specimen is placed close to a point source of x-rays; the...
scanning electron microscopy
Scanning electron microscopy (SEM) is an advanced imaging technique used in microscopy to obtain high-resolution,...
fluorescence lifetime imaging
Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of...
calcium indicator
A calcium indicator is a molecule that is sensitive to changes in calcium ion (Ca2+) concentrations in biological systems....
tip-enhanced Raman scattering
Tip-enhanced Raman scattering (TERS) is a powerful technique that combines Raman spectroscopy with the enhanced spatial...
spatial resolution
Spatial resolution refers to the level of detail or granularity in an image or a spatial dataset. It is a measure of the...
whole slide imaging
Whole slide imaging (WSI), also known as virtual slide microscopy or digital pathology, is a technology that involves the...
address (memory)
Optical addressability refers to the capability to control or manipulate individual elements that emit or modulate light...
ultraviolet microscopy
The study and photographing of microscope specimens in ultraviolet light; using an optical microscope containing fluorite...
Shack-Hartmann wavefront sensor
The Shack-Hartmann wavefront sensor is an optical device used for measuring the wavefront aberrations of an optical system....
nanoparticle photonic resonator absorption microscopy
Nanoparticle photonic resonator absorption microscopy is a microscopy technique that combines the principles of photonic...
microlens array
A microlens array is a grid-like structure composed of numerous small lenses, typically arranged in a regular pattern. Each...
vertical transmitted illumination
With respect to microscopy, light that is directed through the specimen by a substage condenser.
single-photon avalanche diode
A single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical...
acousto-optics
Acousto-optics is a branch of physics that deals with the interaction of sound waves (acoustic waves) and light waves...
acousto-optic tunable filter
An acousto-optic tunable filter (AOTF) is a device that utilizes the acousto-optic effect to selectively filter and transmit...
deep multiphoton microscopy
Deep multiphoton microscopy is an advanced imaging technique used in biological and biomedical research to visualize...
multiline laser system
A multiline laser system refers to a type of laser that is capable of emitting multiple discrete wavelengths or spectral...
apochromat
An apochromat, often referred to as an apochromatic lens or apochromatic objective, is a type of optical system designed to...
transmission electron microscope
A transmission electron microscope (TEM) is a powerful microscopy technique that uses a beam of electrons to create...
photoelectron microscopy
Surface analysis by means of photon induced electron emission. PEM methods provide high lateral resolution of the observed...
laser crystal
A laser crystal, also known as an active medium or gain medium, is a solid-state material used in the construction of...
photon-rich
Photon-rich typically refers to an environment or situation where there is an abundance of photons present. In the context...
micro-optics
Micro-optics refers to the design, fabrication, and application of optical components and systems at a microscale level....
birefringence
Birefringence is an optical property of certain materials that causes them to exhibit different refractive indices for light...
differential interference contrast microscopy
Differential interference contrast (DIC) microscopy, also known as Nomarski interference contrast microscopy, is an optical...
autofluorescence
Autofluorescence refers to the natural emission of fluorescence exhibited by certain biological structures or molecules when...
working distance
In microscopy, the clear distance between the specimen being viewed and the first optical element of the objective lens.
fluorescent protein
Fluorescent proteins are proteins that exhibit the property of fluorescence, which is the ability to absorb light at a...
phase contrast microscopy
Phase contrast microscopy is an optical microscopy technique that enhances the contrast of transparent and colorless...
photon sieve
A photon sieve is an optical device used in the field of optics and imaging. It's designed to focus and shape light,...
optical filter
An optical filter is a device that selectively transmits or blocks specific wavelengths, or colors, of light. Optical...
magnetorheological finishing
Magnetorheological finishing (MRF) is a precision optics polishing technique used for shaping and finishing optical surfaces...
GRIN lens
A GRIN (gradient index) lens is a type of optical lens that utilizes a gradient in refractive index across its volume rather...
fluorescent screen
A fluorescent screen refers to a phosphorescent or fluorescent-coated surface that emits visible light when exposed to other...
acousto-optic deflector
An acousto-optic deflector (AOD) is a type of device that utilizes the acousto-optic effect to control the direction of...
bright-field illumination
The illumination generally used in microscopy, whereby the specimen appears dark against a light background.
optics
Optics is the branch of physics that studies the behavior and properties of light, including its interactions with matter...
ultraviolet fiber optics
Ultraviolet (UV) fiber optics refers to optical fibers that are designed and optimized for the transmission of ultraviolet...
fiber optic imaging bundle
A fiber optic imaging bundle is a specialized optical device composed of multiple optical fibers bundled together. Each...
near-infrared camera
A near-infrared (NIR) camera is an imaging device designed to capture images in the near-infrared region of the...
holographic microscopy
The holographic recording of a microscopic specimen whereby magnification is achieved by alteration in a wavelength or...
axicon
An axicon is a type of optical component characterized by its conical shape and its ability to transform a collimated...
Abbe condenser
An Abbe condenser is a type of optical component used in microscopy to enhance the illumination of the specimen. Named after...
beamsplitter
A beamsplitter is an optical device used to divide a beam of light into two or more separate beams, typically by reflecting...
electron multiplication charge-coupled device camera
An EMCCD (electron-multiplying charge-coupled device) camera is a type of scientific camera specifically designed for...
adaptive deconvolution
Adaptive deconvolution is a computational method that aims to improve the resolution and fidelity of signals or images that...
second-harmonic generation microscopy
A nonlinear label-free imaging technique commonly used during surgical procedures for the visualization of collagen fibers...
blue diode laser
A blue diode laser is a type of semiconductor laser that emits light in the blue wavelength range of the electromagnetic...
diffrimicroscopy
lensless microscopy
Lensless microscopy, also known as computational or holographic microscopy, is an imaging technique that captures and...
stereomicroscopy
The use of a specialized optical microscope designed to provide a more three-dimensional view of a sample. Stereomicroscopy...
spatial light interference microscopy
Spatial light interference microscopy (SLIM) is an optical microscopy technique that belongs to the category of quantitative...
cardioid condenser
An oil immersion condenser used to permit only light that has been diffracted or dispersed by a microscope specimen to enter...
Nomarski microscopy
Also referred to as differential interference contrast (DIC) microscopy; Nomarski microscopy is a unique form of microscopy...
fiber laser
A fiber laser is a type of laser in which the active gain medium is an optical fiber doped with rare-earth ions such as...
modulated transfer function
The modulated transfer function (MTF) is a measure used in optical engineering and imaging science to describe the ability...
optical parametric amplification
Optical parametric amplification (OPA) is a process in nonlinear optics where a weak signal beam is amplified by a much...
volumetric imaging
Volumetric imaging refers to the capture, visualization, and analysis of three-dimensional (3D) information from a volume of...
lateral shearing interferometer
A lateral shearing interferometer is an optical device used in interferometry to measure phase differences between two beams...
ultraviolet lens
A microscope used either to detect selective absorption of various wavelengths by the specimen or to achieve increased...
carbon film
In analysis, the carbon layer that is evaporation-deposited on a specimen to protect and ready it for study by electron...
superresolution
Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by...
femtosecond laser
A femtosecond laser is a type of laser that emits ultrashort pulses of light with durations on the order of femtoseconds,...
oblique illumination
A common technique in microscopy, oblique illumination is one in which the object is illuminated by a light source that is...
harmonic generation
Harmonic generation refers to a nonlinear optical process in which incoming photons interact with a material and produce new...
high-content screening
Also known as HCS, an analytical method designed to collect statistically relevant amounts of quantitative data on many...
tip-enhanced Raman spectroscopy
A spectroscopic technique that combines the high chemical specificity of Raman scattering and signal sensitivity provided by...
ball lens
A ball lens is a small, spherical optical component typically made of glass or other transparent materials. It is...
vortex phase plate
A vortex phase plate is an optical device designed to impart a phase singularity, commonly referred to as a vortex or phase...
random access multiphoton microscopy
Also known as RAMP microscopy, random access multiphoton microscopy is a microscopic technique that uses multiple...
time-correlated single photon counting
Time-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the...
galvanometric scanner
A galvanometric scanner, also known as a galvanometer scanner or galvo scanner, is a type of optical scanning device used to...
feret's diameter
In microscopy, the measured distance between theoretical parallel lines that are drawn tangent to the particle profile and...
cresyl violet
Cresyl violet, also known as cresyl violet acetate or cresyl echt violet, is a synthetic organic compound belonging to the...
vertical incident illumination
With respect to microscopy, light that is directed down through the objective onto the specimen and then returned by...
chemical microscopy
The field of microscopy as applied to chemical problems and analysis.
linear positioning stage
A linear positioning stage, also known as a linear stage or translation stage, is a precision mechanical device used to...
direct illumination
Light produced by visible radiation that moves from the light source to the object without reflection. With respect to...
zero-order filtering
The removal of the zero-order component of the Fourier spectrum distribution of an object with a small, opaque absorber or...
azimuthal polarization
Azimuthal polarization refers to a specific polarization state of light where the electric field vector of the...
CCD camera
A CCD camera, or charge-coupled device camera, is a type of digital camera that utilizes a CCD image sensor to capture and...
rhodamine
Rhodamine refers to a family of fluorescent organic dyes that are widely used in various fields, including biology,...
supercontinuum
Supercontinuum refers to a broad spectrum of light that spans a wide range of wavelengths, typically from the visible to the...
photomicrograph
A photomicrograph is an image taken through a microscope, typically using a camera attached to the microscope's eyepiece or...
digital holographic microscopy
Digital holographic microscopy (DHM) is an advanced imaging technique that combines holography and digital image processing...
label-free
Label-free refers to a technique or method that does not require the use of additional labels, tags, or markers to detect or...
dark-field microscopy
A technique whereby the sample is illuminated by a hollow cone of light larger than the acceptance angle of the objective,...
stochastic optical reconstruction microscopy
Stochastic optical reconstruction microscopy (STORM) is a superresolution microscopy technique that enables imaging of...
single molecule localization microscopy
Single molecule localization microscopy (SMLM) is a group of super-resolution microscopy techniques that surpass the...
metasurfaces
Metasurfaces are two-dimensional arrays of subwavelength-scale artificial structures, often referred to as meta-atoms or...
microscopy
Microscopy is a scientific technique that involves the use of microscopes to observe and study objects that are too small to...
chromatic aberration
Chromatic aberration is an optical phenomenon that occurs when different wavelengths (colors) of light are refracted by a...
brightfield
Brightfield refers to a type of microscopy and imaging technique in which the specimen is illuminated with a white light...
intravital microscopy
Intravital microscopy is an imaging technique used to visualize biological processes within living organisms, typically in...
two-photon excited fluorescence
Two-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living...
scanning electron microscope
A scanning electron microscope (SEM) is a powerful imaging instrument used in scientific research, materials...
phase
In optics and photonics, "phase" refers to a property of electromagnetic waves, such as light, that describes the position...
quantitative phase imaging
Quantitative phase imaging (QPI) is an advanced imaging technique used in microscopy to measure and analyze the optical...
dichroic coating
A dichroic coating, also known as a dichroic filter or dichroic mirror, is an optical coating designed to selectively...
chirped mirrors
Chirped mirrors are optical devices designed to manipulate the spectral properties of ultrashort laser pulses. They consist...
motion controller
In optics, a motion controller refers to a device or system that precisely controls the movement of optical components, such...
fluorophore
A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external...
phase-contrast generation
Microscopy technique to convert the phase structure of the wave transmitted or reflected by the specimen into a...
long-pass filter
A long-pass filter is an optical filter that allows longer wavelengths of light to pass through while blocking or...
fluorescence
Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other...
second-harmonic generation
Second-harmonic generation (SHG) is a nonlinear optical process that occurs when two photons with the same frequency combine...
beam shaper
A beam shaper is an optical device or system designed to modify the spatial profile or intensity distribution of a laser or...
magnetic lens
An arranged series of coils, magnets or electromagnets disposed in such a way that the resulting magnetic fields generate a...
scientific complementary metal-oxide semiconductor
Scientific complementary metal-oxide semiconductor (sCMOS) refers to a type of image sensor technology specifically designed...
laser microscopy
Technique using functional optical microscope with the addition of a coherent source collinear with the image path. The...
Förster resonance energy transfer
Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced...
STED microscopy
STED microscopy, or stimulated emission depletion microscopy, is a superresolution imaging technique in fluorescence...
reflection x-ray microscopy
A means of high resolution study through the application of soft and hard x-rays onto a sample surface in order to obtain...
epifluorescence microscopy
Fluorescence imaging technique in which the excitation light from the objective is directed into the sample producing...
laser diode module
A laser diode module is a compact device that incorporates a laser diode, optics, and often other components into a single...
structured illumination microscopy
Structured illumination microscopy (SIM) is an advanced optical imaging technique used in microscopy to enhance the...
scientific CMOS camera
A scientific CMOS (complementary metal-oxide-semiconductor) camera is a type of digital camera specifically designed for...
wide-field fluorescence microscopy
Wide-field fluorescence microscopy uses either naturally occurring structures or staining with fluorescent tags that are...
reflectance confocal microscopy
Reflectance confocal microscopy (RCM) is a non-invasive imaging technique that allows for high-resolution, in vivo imaging...
ultrafast pulsed laser
An ultrafast pulsed laser is a type of laser that emits extremely short pulses of light with durations typically on the...
morphological processing
A set of algorithms used to study the structural and spatial characteristics of objects in a digitized image for...
video-intensified microscopy
The observation of specimens by a microscope linked to a television camera and an image processor.
microscope immersion fluid
The liquid used in microscopy to fill the space between the high-power objective lens and the microscope slide in order to...
x-ray phase contrast microscopy
Used for high-resolution surface study with subnanometer resolution. XRIM uses interfacial phase contrast with application...
photon jet
A photon jet is a narrow, highly collimated beam of light that is formed when light interacts with small dielectric...
nonlinear polarization
Nonlinear polarization refers to the phenomenon where the polarization of a material responds nonlinearly to an applied...
beta fluorography
The use of a short-duration electron beam to record high-speed events that occur in microscopic objects made of materials...
intensified charge-coupled device camera
An intensified charge-coupled device (ICCD) camera is an advanced imaging device that combines the capabilities of both an...
light sheet fluorescence microscopy
Also known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and...
three-photon microscopy
Three-photon microscopy is an advanced imaging technique that belongs to the family of multi-photon microscopy methods. It...
piezoelectric motion systems
Piezoelectric motion systems are mechanical systems that utilize piezoelectric materials to generate controlled motion or...
nanopositioning
Nanopositioning refers to the precise and controlled movement or manipulation of objects or components at the nanometer...
nonlinear optical materials
Nonlinear optical materials are substances that exhibit optical properties that are not linearly proportional to the...
point spread function
The point spread function (PSF) is a fundamental concept in imaging that describes the response of an imaging system to a...
photobleaching
Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently...
aplanatic points
Aplanatic points refer to specific points in an optical system where certain aberrations, such as spherical aberration or...
fiber-based confocal luminescence microscope
A microscope in which laser light is delivered through single-mode fibers that replace the pinhole usually used in confocal...
filovirus
Filoviruses are a family of viruses that are responsible for severe and often fatal hemorrhagic fevers in humans and...
stage micrometer
In microscopy, a calibrated scale on a slide that may be viewed to determine the exact magnification factor of the...
counting chamber
In microscopy, the chamber that is contained on a microscope slide to hold a certain amount of fluid. It is calibrated...
total internal reflection fluorescence
Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the...
fluorescence microscopy
Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to...
indirect illumination
The light formed by visible radiation that, in traveling from light source to object, undergoes one or more reflections. In...

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