Definition of holographic microscopy

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Photonics Dictionary

holographic microscopy: The holographic recording of a microscopic specimen whereby magnification is achieved by alteration in a wavelength or radius of curvature between recording and reconstruction of the wavefront. With this technique the quality and field of the image can surpass those produced by microscopic methods.

A microscopy technique that utilizes optical diffraction tomography (ODT), which enables users to quantitatively and noninvasively investigate the intrinsic properties of cells. ODT reconstructs the 3D distribution of the refractive index and by doing so, provides structural and chemical information of the cell including dry mass, morphology and dynamics of the cellular membrane.

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