Definition of light sheet fluorescence microscopy

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light sheet fluorescence microscopy: Also known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and fast biological processes in vivo. In this method, a light sheet illuminates the specimen from the side in a single focal plane and is detected using a second objective oriented perpendicularly to the light sheet. By focusing light sheets onto a confined volume of the sample through cylindrical optics, optical sectioning and reduced phototoxicity are achieved.

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