Definition of rhodamine

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Photonics Dictionary

rhodamine: Rhodamine refers to a family of fluorescent organic dyes that are widely used in various fields, including biology, chemistry, medicine, and materials science. These dyes are known for their bright fluorescence, photostability, and versatility, making them valuable tools for fluorescence imaging, labeling, and detection applications.

The rhodamine family includes several derivatives, each with its own specific properties and applications. Some common types of rhodamine dyes include:

Rhodamine 6G (Rh6G): Rhodamine 6G is one of the most well-known and widely used members of the rhodamine family. It emits intense green fluorescence when excited by visible light, making it suitable for fluorescence microscopy, flow cytometry, and laser-induced fluorescence detection.

Rhodamine B (RhB): Rhodamine B is another popular rhodamine derivative that emits red fluorescence. It is commonly used as a fluorescent tracer in biological studies, histology, and dye-labeled materials.

Rhodamine 101 (Rh101): Rhodamine 101 is a red-emitting dye with high photostability and brightness. It is used in various applications, including fluorescence imaging, cell labeling, and super-resolution microscopy.

Rhodamine 123 (Rh123): Rhodamine 123 is a green-emitting dye that is widely used as a mitochondrial stain in cell biology and fluorescence microscopy. It selectively accumulates in mitochondria, making it useful for studying mitochondrial function and dynamics.

Rhodamine dyes typically contain a central heterocyclic ring structure with attached aromatic or aliphatic substituents. The fluorescence properties of rhodamine dyes can be modified by changing the chemical structure of the dye molecule, such as by introducing different functional groups or altering the conjugation system.

In addition to their fluorescence properties, rhodamine dyes are also known for their pH sensitivity, solvatochromic behavior, and sensitivity to environmental factors, which can be exploited for various sensing and detection applications.

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