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Features
Getting in Deeper
Thanks to microendoscopic research and technology innovations, patients could be in for a new set of close-ups. Several groups of investigators are working to implement optical biopsies, in which the traditional methods will be replaced by an in situ microscopic examination. The result could be a better handle on disease while patient discomfort and risk are diminished. A look at various efforts shows what progress is being made. Brains at work To the unaided eye, a working brain appears...
BioPhotonics, July 2008
Prospects for Photodynamic Therapy in Dentistry
Photodynamic therapy (PDT) involves the use of a photosensitizer that is activated by illumination in the presence of oxygen. The exposure of the photosensitizer to light results in the formation of toxic oxygen species, thereby causing localized...
BioPhotonics, July 2008
Light Creates Tiny Patterns
The old trick, practiced by schoolboys everywhere, of concentrating a beam of sunlight through a magnifying lens to ignite paper -- or an unfortunate ant -- has been given a new twist. By using a microscopic plastic bead in place of the lens and...
Photonics.com, June 2008
A Bridging Method Expands the Quantum Efficiency of Sensors
The demand for cost-effective, high-performance imaging detectors is prevalent within the scientific research community. CCD sensors are arrays of photosensitive pixels that are covered by parallel layers of polysilicon gates. The layers provide...
BioPhotonics, June 2008
Getting a Better View from the Inside
According to modern medicine, there is a grain of truth in the old adage: The way to a man’s heart is through his stomach. Dr. Michael B. Wallace, a gastroenterologist with the Mayo Clinic in Jacksonville, Fla., uses narrow-diameter and flexible...
BioPhotonics, June 2008
High-Speed Imaging for DNA Sequencing
The key to personalized medicine is understanding the role that an individual’s genes play in his or her health. To reap the benefits of personalized medicine, the billions of nucleotide bases that comprise an individual’s genome must be sequenced —...
BioPhotonics, June 2008
Polarized Crystals Enable Malaria Diagnosis
According to the 2005 World Malaria Report by the World Health Organization and UNICEF, as many as 500 million malaria cases are recorded each year, resulting in at least 1 million deaths. Although a microscopic examination using a special stain has...
BioPhotonics, June 2008
Quantum Dots Move Beyond Fluorescence Imaging
Quantum dots are transforming life sciences imaging because of their extraordinary photostability, brightness, broad excitation, narrow emission, long fluorescence lifetimes and multiplexing capability. The nanometer-scale inorganic crystals contain...
BioPhotonics, June 2008
Can the Miracles Promised by Carbon Nanotubes Be Realized?
By delivering drugs directly to the source of disease, carbon nanotubes and other nanoparticles could reduce the toxicity that chemotherapy agents and other drugs introduce to the body. Of all the nanoparticles, carbon nanotubes promise to...
BioPhotonics, May 2008
Overcoming Optical Challenges to Live-Cell TIRF Microscopy
Total internal reflection fluorescence (TIRF) microscopy is a powerful technique. It provides extremely thin axial sectioning with excellent signal-to-noise ratios, allowing observation of fluorescent events occurring at the interface between two...
BioPhotonics, May 2008
Robust Optics for Microscopy Applications
The evolution of microscopy from direct human observation to digital image capture has significantly advanced research in the life sciences. For microscopy, digital cameras, along with image-processing and data algorithms, have allowed observations...
BioPhotonics, May 2008
Trends in Optical Trapping
The term “optical trapping” is perhaps misleading: It implies little more than grabbing a molecule or other object and possibly transporting it to some other place. But, as is apparent from studies that have emerged recently, optical trapping is a...
BioPhotonics, May 2008
Magnetic Resonance Imaging Moves Ahead
MRI, which allows doctors and clinicians to peer inside the body without the use of ionizing radiation, is particularly useful for imaging soft tissues, which is why athletes often go into a scanner when injured. Recent advances promise to make...
BioPhotonics, April 2008
Nanotechnology and Photonics Join Forces in Biomedicine
In the recent past, biology has shifted from the microscale to the nanoscale, from the level of the cell to that of viruses and of cellular constituents such as proteins, nucleic acids and most organelles. By extension, tools for the investigation...
BioPhotonics, April 2008
Raman Spectroscopy Analysis of Polymorphs
Some molecules with the same chemical formula can have different crystal structure forms — called polymorphs. There are two ways in which crystal structures can arise: arrangement (or packing) polymorphism and conformational polymorphism....
BioPhotonics, April 2008
Raman Spectroscopy Meets Flow Cytometry
In flow cytometry, which performs high-speed optical analysis of single cells, suspensions of cells are hydrodynamically focused single file through a laser beam (Figure 1). The light scatter provides information about gross morphological features...
BioPhotonics, April 2008
Tip-Enhanced Raman Spectroscopy for Bioanalysis
A fundamental goal in the life sciences is to understand how the elemental building blocks (nucleobases, amino acids, etc.) are composed in living organisms. Chemical processes in cells are carried out by macromolecules such as nucleic acid chains,...
BioPhotonics, April 2008
Detecting Colon Cancer, Virtually
For scientists researching ways to prevent death, colon cancer offers a tempting target because screening techniques can spot precancerous tissue, and it is known which part of the population should be checked. And even though benign lesions (known...
BioPhotonics, March 2008
Hyperspectral Imaging for the Life Sciences
Multispectral and hyperspectral imaging techniques were developed for applications in remote sensing and astronomy but have slowly transitioned into use in many areas of the life sciences, including fluorescence microscopy and in vivo imaging....
BioPhotonics, March 2008
The Quest for a Better Biological Imaging System
Imaging biological systems on the cellular and even molecular level requires a material that is as versatile as it is durable. Quantum dots possess many of the attributes necessary for this purpose. The strengths of these nanosize semiconductors...
BioPhotonics, March 2008
LEDs for Fluorescence Microscopy
Until recently, fluorophore excitation has been achieved using broad-spectrum mercury gas discharge lamps with a combination of optical filters to remove many of the unwanted wavelengths. These lamps are widely used and accepted in microscopy...
BioPhotonics, February 2008
New Developments in SPR Biosensing
Surface plasmon resonance (SPR) biosensors have been developed for a variety of applications, from early diagnosis of disease to drug discovery and from water purity analysis to identification of pathogens possibly used for bioterrorism. Offering...
BioPhotonics, February 2008
Raman Analysis Speeds into Biomedicine
Raman is one of a number of spectrographic techniques that may be used to study biological materials. Of these, dispersive Raman imaging offers a compelling range of capabilities, providing superior chemical specificity with excellent spatial...
BioPhotonics, February 2008
CMOS Cameras Enter the Life Sciences Market
When a rat decides on a course of action, you can’t ask it what factors played a role in that decision. But Jason Ritt, a postdoctoral fellow at the McGovern Institute for Brain Research at MIT in Cambridge, Mass., had to get as close as possible to...
BioPhotonics, January 2008
Fiber Optics and MEMS
During the past 16 years, nonlinear optical microscopy has evolved from a photonic novelty to a well-established laboratory tool. Rapid development of fiber optic components with growing functionalities and decreasing size provides enormous...
BioPhotonics, January 2008
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