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Fast EEF Spectroscopy Quantifies Unstable Samples

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Fluorescence spectroscopy is used routinely to analyze all types of luminescent samples, from solid materials for semiconductor devices to fluorescent probes for medical imaging. And in a mixture of many fluorescent compounds, it is possible to identify each one by its combination of excitation and emission wavelengths.

MARIA TESA, EDINBURGH INSTRUMENTS

Excitation-emission fluorescence spectroscopy (EEF) is particularly interesting in materials characterization, environmental science and the food industry. For example, the user can quantify multiple emitting sites in rare-earth doped crystals1 or different types of carbon nanotube structures in a mixture2. This technique is also used to obtain the fingerprint of food samples3, study bacterial activity in environmental samples or characterize the organic matter content in water4. The spectral fingerprint of a fluorescent compound mixture is provided by its excitation-emission map (EEM),...Read full article

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    Published: October 2017
    Glossary
    photobleaching
    Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently reduced or eliminated upon prolonged exposure to light. This process occurs due to the photochemical destruction or alteration of the fluorophore molecules, rendering them non-fluorescent. Key points about photobleaching: Mechanism: Photobleaching is typically a result of chemical reactions induced by the absorbed photons. The excessive light exposure causes the...
    quantum efficiency
    Quantum efficiency (QE) is a measure of the effectiveness with which a device or system, typically in the context of photonics or electronics, converts incoming photons (light) into a useful output signal or response. It is expressed as a ratio or percentage and quantifies the number of electrons or charge carriers generated in response to the incident photons. In other words, quantum efficiency provides a measure of how well a device can capture and utilize photons to produce an electric...
    Excitation-emission fluorescence spectroscopyEEFexcitation-emission mapEEMpolyaromatic hydrocarbonphotomultiplier tubesPMTmonochromatorsphotobleachingphotostabilityphotodegradationCharge Coupled DeviceCCDfluorometersdetectorsquantum efficiencyFeatures

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