Also known as single plane illumination microscopy (SPIM), this process was designed for imaging of sensitive samples and fast biological processes in vivo. In this method, a light sheet illuminates the specimen from the side in a single focal plane...

An automatic scanning instrument that is used to study a substance's fluorescence over a wide range of wavelengths. It...

The technique that uses light to detect and analyze the antibodies produced by a specimen stained with an organic dye.

A type of laser spectroscopy used to measure short-lived isotopes by observing sudden bursts of fluorescence resulting from...

Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other...

An optical recording technique that uses light source(s), a beamsplitting cube, light detector(s) and an optical fiber...

Rhodamine refers to a family of fluorescent organic dyes that are widely used in various fields, including biology,...

Recombinase polymerase amplification (RPA) is a molecular biology technique used to rapidly amplify specific DNA or RNA...

The fluorescent emission spectrum emitted by certain substances following the irradiation of one of these substances with...

An instrument used to measure the duration of fluorescence emanating from a biological sample to monitor and evaluate its...

The light emitted from tissues without the use of fluorescent dyes as markers. Because cancerous tissues and normal tissues...

A nonradiative transition refers to a process in which an electron or an atom undergoes a change in its energy state without...

Fluorescent proteins are proteins that exhibit the property of fluorescence, which is the ability to absorb light at a...

Nanoparticle photonic resonator absorption microscopy is a microscopy technique that combines the principles of photonic...

Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently...

Intravital microscopy is an imaging technique used to visualize biological processes within living organisms, typically in...

Fluorescence produced by a material that has been treated with a dilute solution of fluorescing material.

An APD detector, or avalanche photodiode detector, is a type of photodetector used to detect light or optical signals by...

Twisted intramolecular charge transfer (TICT) is a phenomenon observed in certain organic molecules containing...

A calcium indicator is a molecule that is sensitive to changes in calcium ion (Ca2+) concentrations in biological systems....

In the context of optical materials, the term "impurity level" refers to the presence of foreign atoms or molecules within...

Autofluorescence refers to the natural emission of fluorescence exhibited by certain biological structures or molecules when...

Spatially offset Raman spectroscopy (SORS) is a technique that extends traditional Raman spectroscopy by incorporating...

Superresolution refers to the enhancement or improvement of the spatial resolution beyond the conventional limits imposed by...

Structured illumination microscopy (SIM) is an advanced optical imaging technique used in microscopy to enhance the...

The process in which the normal rate of fluorescent emission from a substance is enhanced by virtue of the optical gain of...

A monochromator is an optical instrument used to isolate and select a narrow range of wavelengths from a broader spectrum of...

The collection and detection of x-rays by virtue of their ionizing properties. The ionization may be perceived directly by a...

Scientific complementary metal-oxide semiconductor (sCMOS) refers to a type of image sensor technology specifically designed...

A method for remote measurement of the temperature of moving surfaces in harsh environments by using a laser to stimulate...

Azobenzene is a chemical compound consisting of two phenyl rings linked by a N=N double bond. It is well-known for its...

Photoluminescence is a phenomenon in which a material absorbs photons (light) at one wavelength and then re-emits photons at...

A single-photon avalanche diode (SPAD) is a specialized type of photodetector designed to detect extremely weak optical...

Metabolic imaging refers to the visualization and quantification of metabolic processes within living organisms, tissues, or...

A multiline laser system refers to a type of laser that is capable of emitting multiple discrete wavelengths or spectral...

An optical filter is a device that selectively transmits or blocks specific wavelengths, or colors, of light. Optical...

Fluorescein is a synthetic organic compound extensively used as a fluorescent tracer in various applications, particularly...

Förster resonance energy transfer (FRET) is a mechanism describing the transfer of energy between two closely spaced...

The spectroscopic study of radiation emitted by the process of fluorescence.

Luminescence is the emission of light that occurs without the involved substance undergoing a significant increase in...

A nitrogen vacancy (NV) refers to a specific type of defect or impurity in a crystal lattice where a nitrogen atom replaces...

A time-to-amplitude converter (TAC) is an electronic circuit that converts a time interval between two events into an...

Deep multiphoton microscopy is an advanced imaging technique used in biological and biomedical research to visualize...

A filter fluorometer that uses a photomultiplier tube to detect the fluorescence of a sample.

Ratiometry is a technique used in various scientific fields, particularly in analytical chemistry and biochemistry, to...

A lab-on-a-chip (LOC) is a miniaturized device that integrates various laboratory functions and capabilities onto a single,...

STED microscopy, or stimulated emission depletion microscopy, is a superresolution imaging technique in fluorescence...

A photomultiplier tube (PMT) is a highly sensitive vacuum tube that detects and amplifies low levels of light. It is widely...

Technique using functional optical microscope with the addition of a coherent source collinear with the image path. The...

Fluorescence formed when atoms of one element collide with excited atoms of another.

Microscopy is a scientific technique that involves the use of microscopes to observe and study objects that are too small to...

Laser-induced fluorescence (LIF) is a spectroscopic technique that involves using a laser to excite molecules or atoms,...

Fluorescence imaging technique in which the excitation light from the objective is directed into the sample producing...

Adiabatic laser colorimetry is a technique used to measure the color of materials based on the principle of adiabatic...

The photographic recording of a subject that exhibits luminescence only for an extremely short time (10-8 s) after the...

In ultraviolet and fluorescence photography, the term applied to the filter used in the photographic system and with the...

Stochastic optical reconstruction microscopy (STORM) is a superresolution microscopy technique that enables imaging of...

Fluorescence microscopy is a specialized optical imaging technique used in biology, chemistry, and materials science to...

A chemical substance that exhibits fluorescence when excited by ultraviolet radiation, x-rays or an electron beam. The...

Laser viewing cards, also known as laser alignment cards or laser safety cards, are specialized cards or sheets designed to...

The use of a short-duration electron beam to record high-speed events that occur in microscopic objects made of materials...

A coverslip, also known as a cover glass or cover slip, is a thin and flat piece of transparent material typically made of...

Medical lasers are devices that produce intense beams of light with specific characteristics and properties, which are used...

A fluorescent screen refers to a phosphorescent or fluorescent-coated surface that emits visible light when exposed to other...

A photodetector, also known as a photosensor or photodiode, is a device that detects and converts light into an electrical...

The study of flames by means of a laser emitting blue light and a spectrometer to measure the green fluorescence created by...

The analysis of fluorescence emitted by discrete atoms, in flames, that have absorbed radiation from an external source. It...

A pump-probe technique whereby a single wavelength source may be used in order to excite a given sample to determine...

A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external...

Time-correlated single photon counting (TCSPC) is a technique used in photon counting applications, particularly in the...

Two-photon excited fluorescence (TPEF) is a nonlinear optical method that allows imaging of biological cells and living...

Fluorescence lifetime imaging (FLIM) is an advanced imaging technique that provides information about the lifetime of...

An intensified charge-coupled device (ICCD) camera is an advanced imaging device that combines the capabilities of both an...

Any fluorescence in which the wavelength of the emitted light differs from that of the absorbed light.

A fiber optic imaging bundle is a specialized optical device composed of multiple optical fibers bundled together. Each...

A dichroic mirror, also known as a dichroic beamsplitter or interference filter beamsplitter, is an optical device that...

An EMCCD (electron-multiplying charge-coupled device) camera is a type of scientific camera specifically designed for...

An instrument used to detect the fluorescence emitted by a source in comparison with a reference.

A fluoroscope equipped with a magnified, fine-grained fluorescent screen to determine the fluorescence emitted by a...

With respect to atomic fluorescence spectroscopy, the fluorescence that is emitted by an atom at a spectral line of a...

Referred to as SWCNTs, these cylindrical nanostructures composed of a folded sheet of graphene can be used as near-infrared...

A long-pass filter is an optical filter that allows longer wavelengths of light to pass through while blocking or...

Spectroscopy is a technique used in the study of interactions between matter and electromagnetic radiation. It involves...

Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the...

In atomic fluorescence spectroscopy, fluorescence emitted by an atom at a spectral line of a wavelength identical to that of...

The optical energy transfer between ions of differing atoms.

The suppression of fluorescence by absorption of the stimulating radiation.

A type of fluorescence spectroscopy in which an excimer laser, a dye laser and a frequency-doubling device are used to...

A fiber optic spectrometer is a device used for measuring the spectral content of light. It utilizes optical fibers to...

The analysis and measurement of the fluorescence emitted by a source. Fluorometric processes are more sensitive than light...

Ultraviolet (UV) fiber optics refers to optical fibers that are designed and optimized for the transmission of ultraviolet...

A beamsplitter is an optical device used to divide a beam of light into two or more separate beams, typically by reflecting...

This type of miniaturized system, referred to as CMOS qPCR, combines microfluidic technology with an ultralow-light CMOS...

Fluorescence lifetime refers to the average time it takes for a fluorophore, a molecule that absorbs light at one wavelength...

Three-photon microscopy is an advanced imaging technique that belongs to the family of multi-photon microscopy methods. It...

An acousto-optic tunable filter (AOTF) is a device that utilizes the acousto-optic effect to selectively filter and transmit...

A blue diode laser is a type of semiconductor laser that emits light in the blue wavelength range of the electromagnetic...

Wide-field fluorescence microscopy uses either naturally occurring structures or staining with fluorescent tags that are...

A laser having a material, such as ruby, that has an energy state structure of three levels: the ground state (1) wherein...

Flow cytometry is a powerful technique used in biology and medicine for the quantitative analysis of the physical and...

Spectral flow cytometry is an advanced flow cytometry technique that expands the capabilities of traditional flow cytometry...

Fluorescence produced as a result of the intrinsic property of the material itself or the doping of the material with trace...

A device, also called a fluorescence lifetime instrument, that measures decay curves of fluorescing samples. It generally...

A powerful method, referred to as FCS, for determining the average diffusion coefficients of fluorescent molecules in...

A scientific CMOS (complementary metal-oxide-semiconductor) camera is a type of digital camera specifically designed for...

This results from the simultaneous absorption of two photons, each having half the energy needed for excitation and...

Single-molecule Förster resonance energy transfer (smFRET) is a specialized technique in biophysics and molecular...