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Features
Multiwavelength TIRF Microscopy Enables Insight into Actin Filaments
Researchers at the University of California, San Francisco (UCSF) are combining multiple laser excitation wavelengths in total internal reflection fluorescence (TIRF) microscopy to investigate the binding dynamics of individual actin filaments. TIRF microscopy provides a unique method of imaging isolated molecules and complexes in vitro. Additionally, the use of sensitive, low-noise cameras enables researchers to study this behavior in real time. A new plug-and-play method of combining
BioPhotonics, September 2015
Supercontinuum Sources Enhance UHR-OCT Methods
Ultrahigh-resolution optical coherence tomography (UHR-OCT) enables micron-scale, cross-sectional and 3D-imaging capabilities. Supercontinuum sources offer the necessary optical bandwidths, spatial coherence and optical power densities — all...
BioPhotonics, September 2015
Sterilization Effects on Optical Fibers for Clinical Use
Optical fibers have a broad range of uses in medical and clinical applications and can be adapted for many invasive and noninvasive procedures. However, any optical fiber to be used inside a human body first must be sterilized. This article reports...
BioPhotonics, September 2015
Lasers Make Their Marks on Skin and Tissue Procedures
Light-based aesthetic procedures are advancing from well-established spa-based procedures to trickier problems, such as resistant tattoo removal and treatments for wounds, scars and skin diseases. Laser hair removal was the number one laser-based...
BioPhotonics, August 2015
Optimizing Experimental Conditions for STED and g-STED Microscopy
The advent of superresolution microscopy has been groundbreaking in its ability to image biological samples, allowing researchers to study previously inaccessible details within cellular processes. New software provides a unique imaging optimization...
BioPhotonics, August 2015
SBF Imaging – Using SEM and LM to Create High-Resolution 3-D Reconstructions
In recent years, there have been considerable advancements in EM-based methods for 3-D reconstructions of large tissue volumes. In this article, we describe a new approach that combines in situ serial sectioning with a unique, multienergy...
BioPhotonics, August 2015
Laser World of Photonics
Laser Munich will focus on image processing, from products and systems to manufacturing and applications. Image processing and laser systems are ever-present in modern manufacturing. The 2015 Laser World of Photonics conference and trade fair,...
Photonics.com, June 2015
Single-Molecule Localization Microscopy with sCMOS Cameras
EMCCD-based cameras with high quantum efficiencies and low readout noise characteristics traditionally have been the preferred technology for SMLM superresolution imaging. However, with tailored localization algorithms, sCMOS cameras have become a...
BioPhotonics, June 2015
Spectroscopy Aids Disease Detection, Faces Obstacles
Spectroscopy advances are steadily reducing disease-detection time frames. These new methods also hone the accuracy of disease identification and provide for more timely treatment protocols. As with anything groundbreaking, though, roadblocks...
BioPhotonics, June 2015
Nanodiamonds Shine New Light on Bio Applications
Defect complexes in nanodiamond particles, which consist of vacancies trapped by nitrogen atoms, form different color centers depending on the nitrogen state present in the diamond. New research into these color centers indicates that nanodiamond...
BioPhotonics, June 2015
Femtosecond Pulses: Control Is Key to New Discoveries
From microscopy to micromanipulation, femtosecond pulses are broadening their reach throughout the photonics research world. To fully realize their potential, precise pulse control is critical. Ultrafast lasers have transformed the worlds of...
BioPhotonics, June 2015
Advances in Optical Filters Provide Rapid, On-the-Go Diagnosis
When it comes to diagnostics, one of the most crucial – and often overlooked – components is the optical filter. But as manufacturing processes improve, optical filters of exceptional quality are being created, which not only deliver...
BioPhotonics, April 2015
Comparing Micromanipulators for Electrophysiology
Several different technologies offer a range of performance options within the confines of an optical microscope for patch-clamp studies, intracellular recording and other demanding experiments in electrophysiology. Electrophysiology encompasses the...
BioPhotonics, April 2015
Focus-Tunable Lenses Enable 3-D Microscopy
Focus-tunable lenses speed up and enhance three-dimensional imaging capabilities in a diverse set of modern microscopy methods including confocal, two-photon and light-sheet microscopy. Microscopy beginners may be perplexed when they notice that...
BioPhotonics, April 2015
Lasers for Microscopy: Major Trends
Laser development for the microscopy market continues to be driven by key trends in applications, which currently include superresolution techniques, multiphoton applications in optogenetics and other areas of neuroscience, and even a shift in...
BioPhotonics, April 2015
Femtosecond Lasers Provide a Gentler Way to Micromachine
From the unbreakably hard to the vulnerably soft – from glass, metals and ceramics to plastics and biological tissue – femtosecond lasers are being increasingly adopted for micromachining a range of materials. Femtosecond lasers are...
Photonics.com, March 2015
Lasers, Optics Enhance Optogenetics Studies
A guide to suitable light sources and fiber optic components for optogenetics research, with a specific emphasis on rodent behavior. The use of lasers and optics for optogenetics is only in its infancy. In the next decade, we can expect researchers...
Photonics.com, March 2015
Diverse Applications Drive Lasers for Multiphoton Microscopy
After years of domination by a single ultrafast laser technology (Ti:S) and its associated add-ons, laser manufacturers have broadened their technology portfolios to better support a diversified and dynamic nonlinear imaging applications space.
BioPhotonics, March 2015
Raman Spectroscopy and Microscopy Enable Life Science Discoveries
The first article in this two-part series looked at Raman spectroscopy applications in tumor surgery, endoscopy and bacterial identification. This second part examines applications in cell sorting, laser trapping, biology probing and more.
BioPhotonics, March 2015
Lens-Free Microscopy Goes Scalable
A compact lens-free microscope could replace microscopes – and even expand their use – in a number of key biomedical applications, such as stem-cell culturing or in vitro drug testing. Using photonic integration, the microscope can even...
BioPhotonics, March 2015
Multiphoton Imaging Takes New Directions
Recent evolutions of ultrafast laser technology uniquely overcome key challenges in bioimaging, especially for long-term in vivo imaging, deep penetration and leveraging multimodal imaging strategies. Since the early 1990s – and even more so...
BioPhotonics, March 2015
Lasers, Optics Enhance Optogenetics Studies
A guide to suitable light sources and fiber optic components for optogenetics research, with a specific emphasis on rodent behavior. The use of lasers and optics for optogenetics is only in its infancy. In the next decade, we can expect researchers...
BioPhotonics, January 2015
Line-Narrowed Laser Module Enables Spin-Exchange Optical Pumping
The optical technique enhances MRI technology for diagnosis and other applications. In the past several decades, medical imaging has made huge strides. The field of MRI in particular has seen huge advancements in technical capability, as recognized...
BioPhotonics, January 2015
Microspectroscopy Enables Real-Time Imaging of Singlet Oxygen
A new experimental setup allows researchers to monitor a highly reactive species of molecular oxygen that plays an important role in a wide range of biological processes. Oxygen is one of the most important molecules in maintaining life, as well as...
BioPhotonics, January 2015
Raman Spectroscopy and Microscopy: Solving Outstanding Problems in the Life Sciences
This first article in a two-part series will examine Raman spectroscopy applications in tumor surgery, endoscopy, bacterial identification and more. The second part will cover applications in cell sorting, laser trapping and probing biology. Raman...
BioPhotonics, January 2015
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