Search
Menu
Opto Diode Corp. - Opto Diode 10-24 LB

Multimodal Label-Free Imaging Drives Biomedical Research

Facebook X LinkedIn Email
James Lopez and Yiwei Jia, Olympus America, Inc.

Label-free noninvasive, nondestructive optical microscopy using multiple simultaneous techniques allows researchers to observe diverse life processes in real time. Optical microscopy is a powerful tool in bioscience research, allowing researchers to observe multiple complex physiological processes in real time. Fluorescence imaging – a major technique used with optical microscope systems – presents many challenges, however. Achieving sufficient concentration of a fluorescent probe within a tissue can be difficult. Phototoxicity caused by singlet oxygen formation is damaging...Read full article

Related content from Photonics Media



    Articles


    Products


    Photonics Handbook Articles


    White Papers


    Webinars


    Photonics Dictionary Terms


    Media


    Photonics Buyers' Guide Categories


    Companies
    Published: September 2012
    Glossary
    coherent anti-stokes raman scattering
    A technique whereby two laser beams, one at an excitation wavelength and the second at a wavelength that produces Stokes Raman scattering, interact coherently in a sample, producing a strong scattered beam at the anti-Stokes wavelength.
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    phototoxicity
    Phototoxicity refers to the harmful effects caused by exposure to light, particularly intense or ultraviolet (UV) light, on living cells or organisms. This phenomenon is often associated with the interaction of light with certain substances, known as photosensitizers, which can lead to cellular damage or death. Phototoxic reactions can occur in various biological systems, including cells, tissues, and organisms. Key points about phototoxicity include: Photosensitizers: Photosensitizers are...
    second-harmonic generation
    Second-harmonic generation (SHG) is a nonlinear optical process that occurs when two photons with the same frequency combine within a nonlinear material, resulting in the generation of a new photon with twice the frequency (and therefore half the wavelength) of the original photons. This phenomenon is a specific case of second-order nonlinear optical effects. Key points about second-harmonic generation include: Nonlinear optical process: SHG is a nonlinear optical effect, meaning that the...
    Adrian Pegorarobiomedical researchBiophotonicsbioscience researchcancerCARScoherent anti-Stokes Raman scatteringFeaturesfemto-CARSfluorescencefluorescent probeImagingintrinsic two-photon excitation fluorescenceIR lasersJames LopezJi-Xin Chenglabel-free imaginglaser-scanning microscopeMicroscopymultimodal imagingmultiphoton excitationOlympusOlympus Americaoptical microscopyPaolo ProvenzanoPhototoxicitysecond-harmonic generationSensors & DetectorsSHGThuc T. LeYiwei Jia

    We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.