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Multiphoton Microscopy Evolves with the Aid of Laser Systems
Multiphoton excitation (MPE) microscopy has successfully transitioned from the demonstration of its potential that took place more than 30 years ago to a powerful imaging method used throughout the life sciences. This breadth is reflected in a range of modern techniques, including two-photon (2P) or three-photon (3P) excitation fluorescence microscopy, coherent anti-Stokes Raman scattering, and harmonic generation microscopy. This technology has been applied to a range of applications from...
BioPhotonics, Nov/Dec 2023
Fluorescence Imaging Could Guide Tissue Infection Diagnosis and Treatment
Necrotizing soft-tissue infections (NSTIs) are aggressive and rapidly advancing infections with a mortality rate between 20% and 30%. In the mainstream media, this disease process is more commonly referred to as “necrotizing fasciitis” or...
BioPhotonics, Nov/Dec 2023
Illuminating the Unseen: Data at Depth with Light-Sheet Fluorescence Microscopy
Light-sheet fluorescence microscopy, also called single plane illumination microscopy (SPIM) is a noninvasive 3D imaging method that employs a sheet of light to illuminate a thin plane of a specimen. This technique reduces out-of-focus light and...
BioPhotonics, Nov/Dec 2023
Live-Cell Imaging Follows Early Signs of Mutation and Disease
Scientists are increasingly turning to live-cell imaging to track changes at the developmental stage of life, which is vital for the advancement of cellular biology, and to grow and monitor proliferating stem cells and cancer cells in a contained...
BioPhotonics, Nov/Dec 2023
Fiber Optic Probes Help Customize Spectroscopic Diagnosis
Diffuse reflection spectroscopy and fluorescence spectroscopy are having a major effect in medicine and the life sciences, with expanding application in physics, chemistry, biology, and medicine. And fiber optic-based probes are becoming an...
BioPhotonics, Sep/Oct 2023
Illumination Advancing Fluorescence Microscopy in Life Sciences, Medical Realms
In many academic research and medical settings, microscopy and imaging have transitioned from using traditional lamp illumination, such as mercury and Xenon lamphouses, to solid-state LED technologies. The benefits of moving to LEDs include long...
BioPhotonics, Sep/Oct 2023
QCL-IR Powers Photoacoustic Spectroscopy in Translational Medicine
Mid-infrared (MIR) optoacoustic spectroscopy is a well-established technique that is currently being leveraged with modern instrumentation to push boundaries in life sciences research and medical diagnostics. Researchers recently demonstrated...
BioPhotonics, Sep/Oct 2023
Build Versus Buy: Considerations in SD-OCT System Design
Many researchers and product developers using spectral-domain optical coherence tomography (SD-OCT) for biomedical research and clinical treatment choose to build their own system. This requires multiple optical and mechanical components, an...
BioPhotonics, Sep/Oct 2023
Fluorescence Polarization Approach Allows Quantitative Cancer Diagnosis
Fluorescence polarization (Fpol) is an optical phenomenon that characterizes the polarization of fluorescence emissions relative to the polarization state of the excitation light. It is sensitive to factors that influence the rotational motion of...
BioPhotonics, Jul/Aug 2023
Precision Positioning Aids Microscopes with Tracking Viral Progression
The rapid spread of COVID-19 and other diseases has shown that understanding viral infection is critical to the health of people around the world. In particular, understanding the dynamics of the earliest stages of infection is important for...
BioPhotonics, Jul/Aug 2023
Quantitative Phase Imaging Advances Regenerative Medicine
Regenerative medicine is defined as the process of replacing, engineering, or regenerating human or animal cells, tissues, or organs to restore or establish their normal function. Researchers have developed techniques and devised innovative...
BioPhotonics, Jul/Aug 2023
Hyperspectral Imaging Allows the Simultaneous Measurement of Fluorophores
Hyperspectral imagers provide an image that produces a detailed spectrum for every pixel, capturing very minute details in a sample. Typically, this technology has been used to measure the spectral reflectance of materials such as agricultural...
BioPhotonics, Jul/Aug 2023
Pulsed Lasers Make Headway in Treating Cardiovascular Disease
Patients with heart disease often face a daunting journey from diagnosis to treatment. They may learn, after experiencing chest pains or shortness of breath, that they have arteriosclerosis, or damaged arteries, through a test such as a coronary...
BioPhotonics, May/Jun 2023
Intravascular Near-Infrared Spectroscopy Predicts Heart Attacks
Lipoproteins carry cholesterol through the closed-looped vascular hematological highway of the human body. This cholesterol can embed itself into the artery walls at varying rates, depending on a multitude of factors, such as anatomical influences...
BioPhotonics, May/Jun 2023
Catheter Use Propels Optical Coherence Tomography in Interventional Cardiology
Optical coherence tomography (OCT) is an interferometric imaging technique that uses light in the near-infrared spectrum to provide detailed, cross-sectional images of biological tissue. OCT is therefore widely used for clinical ophthalmology and...
BioPhotonics, May/Jun 2023
Intravital Multiphoton Microscopy Captures Dynamics of the Beating Heart
Most people know the heart pumps blood to the body, but they may not realize that the blood that fills the four chambers of the heart provides no sustenance to the myocardium, or the heart muscle, itself. Instead, a separate blood supply, the...
BioPhotonics, May/Jun 2023
Light Therapy Could Soon Treat Cardiac Arrhythmia
Heart disease is the leading cause of death in the developed world. Sudden cardiac death is triggered by the breakdown of a normal periodic heart rhythm, also known as the sinus rhythm. The emergence of aberrant, high-frequency sources of electrical...
BioPhotonics, May/Jun 2023
High-Speed Photoacoustic Microscopy Captures Processes Within Tissue
When Alexander Graham Bell discovered the photoacoustic effect in the 1880s, he might not have envisioned that this technology would ultimately be successfully implemented in biomedical imaging applications such as neuroscience, dermatology, and...
BioPhotonics, Mar/Apr 2023
Aptamers Provide Instant Photonics-Based Diagnostics
Aptamer-based diagnostics is a new field in the development of technologies for instantly identifying disease. Aptamers are short sequences (oligonucleotides) of DNA or RNA that bind to a specific target molecule. They are typically 20 to 60...
BioPhotonics, Mar/Apr 2023
Raman Spectroscopy Identifies Disease Characteristics and In Vitro Structure
Since the invention of lasers and their integration into Raman spectroscopy systems, the spectroscopy technique has grown in popularity for use in life science and medical applications as a way to noninvasively analyze a sample and identify the...
BioPhotonics, Mar/Apr 2023
Dynamic Light-Scattering Method Could Guide Alzheimer’s Diagnosis and Treatment
The early detection of neurologic damage caused by Alzheimer’s disease, before symptoms have appeared, is integral to the development of effective treatments. Early detection, made possible by leveraging dynamic light scattering to identify subtle...
BioPhotonics, Mar/Apr 2023
Advanced Holography Efficiently Illuminates Neuronal Patterns
Demystifying the complexity of the human brain is one of the last frontiers in diagnostics and therapeutics. When the brain is sufficiently understood by clinicians, the benefits for human well-being — in the form of cures and treatments for...
BioPhotonics, Jan/Feb 2023
Top Hat Illumination Provides Even Light Distribution Across Samples
Even illumination patterns are of great importance when applied to biomedical imaging because they prevent lighting artifacts that could distort the picture of the sample being analyzed. Therefore, suppliers of microscopy solutions have made the...
BioPhotonics, Jan/Feb 2023
Optical Photothermal IR Spectroscopy Targets Single-Cell Metabolomics
Due to its unique molecular fingerprinting capability, infrared spectroscopy has been widely used to interrogate the overall biochemistry of biological systems. Over the years, this capability has proved the technique’s worth as a powerful...
BioPhotonics, Jan/Feb 2023
Acousto-Optic Components Overcome Limitations in Confocal Microscopy
Confocal and multiphoton microscopy are commonly used to provide 3D-resolved images of in vitro or in vivo tissue samples without physical sectioning. But there are also inherent trade-offs in the use of these techniques when it comes to imaging...
BioPhotonics, Jan/Feb 2023
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