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Technique Enables In Vivo Vascular Imaging at Single-Cell Resolution

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A two-photon fluorescence imaging method developed by researchers at the University of Hong Kong and the University of California, Berkeley was able to image the flow of individual blood cells at 1000 2D frames and 1,000,000 line-scans per second in the brains of awake mice. Such rapid measurements go beyond the reach of conventional two-photon microscopy methods to enable the study of blood flow at the single-cell level in large blood vessels. The ultrafast two-photon fluorescence imaging technique could help scientists better understand how energy is distributed and regulated in both...Read full article

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    Published: September 2022
    Glossary
    fluorescence
    Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
    Research & TechnologyeducationAsia-PacificAmericasUniversity of Hong KongUniversity of California BerkeleyMicroscopytwo-photon microscopyneuroimagingin vivo imagingImagingLight SourcesOpticsTest & MeasurementBiophotonicsmedicalcancerhemodynamicsfluorescenceBioScan

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