MIT researchers have created a detector based on fluorescing carbon nanotubes that is so sensitive it can sense a single molecule of an explosive. To create the sensors, Michael Strano and his colleagues at the institute coated carbon nanotubes with protein fragments normally found in bee venom. This is the first time those proteins have been shown to react to explosives, specifically a class (known as nitro-aromatic compounds) that includes TNT. A sensor created at MIT uses carbon nanotubes (in yellow) covered in protein fragments to detect even a single molecule of an explosive, such as the TNT molecule shown here. (Image: MIT) If developed into commercial devices, such sensors would be far more sensitive than existing explosives detectors – commonly used at airports, for example – that use spectrometry to analyze charged particles as they move through the air. "Ion mobility spectrometers are widely deployed because they are inexpensive and very reliable. However, this next generation of nanosensors can improve upon this by having the ultimate detection limit, [detecting] single molecules of explosives at room temperature and atmospheric pressure," Strano said. Strano has filed for a patent on the technology, which makes use of protein fragments called bombolitins. "Scientists have studied these peptides, but as far as we know, they've never been shown to have an affinity for and recognize explosive molecules in any way," he said. In recent years, Strano's lab has developed carbon nanotube sensors for a variety of molecules, including nitric oxide, hydrogen peroxide and toxic agents such as the nerve gas sarin. Such sensors take advantage of carbon nanotubes' natural fluorescence by coupling them to a molecule that binds to a specific target. When the target is bound, the tubes' fluorescence brightens or dims. The new explosives sensor works in a slightly different way. When the target binds to the bee-venom proteins coating the nanotubes, it shifts the fluorescent light's wavelength, instead of changing its intensity. The researchers built a new type of microscope to read the signal, which can't be seen with the naked eye. This type of sensor, the first of its kind, is easier to work with because it is not influenced by ambient light. "For a fluorescent sensor, using the intensity of the fluorescent light to read the signal is more error-prone and noisier than measuring a wavelength," Strano said. The system in action. (Image: Ardemis Boghossian and Daniel Heller) Each nanotube-peptide combination reacts differently to different nitro-aromatic compounds. By using several different nanotubes coated in various bombolitins, the researchers can identify a unique fingerprint for each explosive they might want to detect. The nanotubes also can sense the breakdown products of such explosives. "Compounds such as TNT decompose in the environment, creating other molecule types, and those derivatives could also be identified with this type of sensor," Strano said. "Because molecules in the environment are constantly changing into other chemicals, we need sensor platforms that can detect the entire network and classes of chemicals, instead of just one type." The researchers also showed that two pesticides that are nitro-aromatic compounds can be detected by the nanotubes as well, making them potentially useful as environmental sensors. The technology has already drawn commercial and military interest. For the sensor to become practical for widespread use, it would have to be coupled with a commercially available concentrator that would bring any molecules floating in the air in contact with the carbon nanotubes. "It doesn't mean that we are ready to put these onto a subway and detect explosives immediately. But it does mean that now the sensor itself is no longer the bottleneck," Strano said. "If there's one molecule in a sample, and if you can get it to the sensor, you can now detect and quantify it." For more information, visit: www.mit.edu