Search
Menu
Excelitas Technologies Corp. - X-Cite Vitae LB 11/24

STED Microscopy: A New Chapter in Light Imaging

Facebook X LinkedIn Email
Marie Freebody, Contributing Editor, [email protected]

When Stefan Hell invented a technique that pushed beyond the diffraction limit of light for the first time, a new field of nanoscale imaging was born. Now laboratories around the world are using STED microscopy to smash through more boundaries in fundamental science. Looking back over two decades of progress, the man behind the revolutionary light imaging technique says he is very satisfied with how STED (stimulated emission depletion) microscopy has evolved our understanding of biomolecules as well as transformed the way we think about how light behaves. “As a result of this...Read full article

Related content from Photonics Media



    Articles


    Products


    Photonics Handbook Articles


    White Papers


    Webinars


    Photonics Dictionary Terms


    Media


    Photonics Buyers' Guide Categories


    Companies
    Published: September 2013
    Glossary
    fluorescence correlation spectroscopy
    Fluorescence correlation spectroscopy (FCS) is a powerful analytical technique used to study the dynamics and interactions of fluorescently labeled molecules in solution at the single-molecule level. It provides information about molecular diffusion, concentration, binding kinetics, and molecular interactions with high sensitivity and temporal resolution. Principle: FCS is based on the analysis of fluctuations in the fluorescence intensity emitted by fluorescently labeled molecules as...
    sted microscopy
    STED microscopy, or stimulated emission depletion microscopy, is a superresolution imaging technique in fluorescence microscopy that surpasses the diffraction limit, enabling the visualization of structures at the nanoscale level. This technique was developed to overcome the limitations imposed by the diffraction of light, which traditionally hindered the resolution of optical microscopy to a few hundred nanometers. Key features and principles of STED microscopy: Superresolution: STED...
    stochastic optical reconstruction microscopy
    Stochastic optical reconstruction microscopy (STORM) is a superresolution microscopy technique that enables imaging of biological specimens at resolutions beyond the diffraction limit of conventional optical microscopy. It falls under the category of single-molecule localization microscopy (SMLM) methods. STORM was first introduced in 2006 and has since become a powerful tool in biological research for visualizing fine details of cellular structures. The key principle behind STORM involves...
    Basic ScienceBiophotonicscell biologyChristian Eggelingcontinuous-wave stimulated emission depletioncorrelation spectroscopyCW-STED microscopyDengue fever virusDENVenergyFeaturesfluorescence correlation spectroscopygreen fluorescent proteinground state depletion imagingGSDIMHCVHepatitis C virusHIVhuman immunodeficiency virusImaginginfluenza virusJochen SieberLeica Microsystems GmbHlive brain imagingMarie Freebodymaterial sciencesMax Planck Institute for Biophysical ChemistryMembrane researchNature MethodsPALMPhoto Activated Localization MicroscopyphotoswitchingRESOLFTsaturated structured-illumination microscopySSIMSTED microscopySTED superresolutionSTED-FCSStefan Helstimulated emission depletion microscopystochastic optical reconstruction microscopySTORMyellow fluorescent protein

    We use cookies to improve user experience and analyze our website traffic as stated in our Privacy Policy. By using this website, you agree to the use of cookies unless you have disabled them.