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Molecular Spectroscopy: Choosing the Right Instrument for the Job

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Lynn Savage, Features Editor, [email protected]

As a field, molecular spectroscopy is as wide as the Mississippi River and as deep as the Mariana Trench. Alone or in conjunction with liquid and gas chromatography instruments, a good spectroscopy system can be used to explore DNA and RNA as well as proteins of various shapes and sizes. It can be used to measure the growth of bacterial cultures and to analyze enzyme kinetics. The right technique will let you nail down the conformation of a protein; the wrong one will leave you baffled as to why your data results aren’t what you expected. In molecular analysis, absorption,...Read full article

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    Published: July 2011
    Glossary
    absorption spectroscopy
    Absorption spectroscopy is a fundamental analytical technique used to study the interaction between electromagnetic radiation and matter. It involves measuring the absorption of light by a sample across a range of wavelengths or frequencies. This absorption is caused by the sample's ability to absorb certain wavelengths of light, which corresponds to the excitation of electrons or molecules to higher energy levels. Principle of absorption: Absorption occurs when the energy of incident...
    chromatography
    The chemical method of separating compounds dissolved in one phase (usually mobile) through its equilibration with a second phase (usually stationary). The mechanism of separation may involve partition, adsorption, permeation or exclusion, or ion exchange.
    emission spectroscopy
    Emission spectroscopy is a technique used to study the emission of electromagnetic radiation (light) by atoms, molecules, or ions when they undergo transitions from excited states to lower energy states. This technique provides valuable information about the energy levels, electronic structure, and chemical composition of the emitting species. Principle of emission: Emission occurs when atoms, molecules, or ions in an excited state return to lower energy states, releasing energy in the form...
    fluorescence spectroscopy
    The spectroscopic study of radiation emitted by the process of fluorescence.
    molecular spectroscopy
    Spectrum analysis concerned with the spectra formed by transitions in molecules.
    absorption spectroscopyAgilent TechnologiesAndor TechnologyAvantesB&W TekbayspecBiophotonicsBristol InstrumentsBruker OpticsCCDchromatographyCMOSD. Michael Olivedefenseemission spectroscopyFeaturesfluorescence spectroscopyFoss NIRSystemsFourier-transform infrared spectroscopyFTIRhamamatsuHoriba ScientificImagingimaging spectroscopyInterspectrumJascoLambda SolutionsLi-Cor BiosciencesMcPhersonmolecular spectroscopynear-IR spectroscopyOcean OpticsPerkinElmerPhoton etc.Princeton Instrumentsreflection spectroscopySensors & DetectorsShimadzu ScientificSpectrometersspectrophotometersspectroscopyStellarNettec5Test & MeasurementThermo Fisher ScientificUV spectroscopyVarian

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