Seeing Deeper with Multiphoton Microscopy
Multiphoton imaging has become a widespread and successful technique for high-resolution 3-D imaging of sensitive living samples, thick tissue sections and even of living animals. However, scientists using the technique are always looking to image deeper, even in highly scattering tissues.
In the past few years, the average power delivered by ultrafast pulsed Ti:sapphire lasers, which are most commonly used in this application, has increased. This capability has enabled deeper tissue...
BioPhotonics, January 2007