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Taking Multiphoton Imaging to New Depths
New laser platforms developed to support the latest needs of biological imaging are poised to fuel the next wave of innovation in multiphoton microscopy: imaging deeper into tissue. Two-photon excited fluorescence (TPEF) microscopy has seen rapid growth in the past two decades, fueled by substantial advances in the supporting laser technology. TPEF microscopy was developed for live-cell imaging and uses femtosecond laser pulses for two-photon excitation of fluorophores. This excitation occ...
BioPhotonics, October 2011
Fluorescence Imaging Progressing from Cells to Tissue
Tissues and even whole animals are not as easily captured with fluorescence imaging as are cells, but recent research and technological developments could change that. Fluorescence imaging has become one of the most powerful tools for studying...
BioPhotonics, September 2011
Intrinsic Fluorescence Lights Up Cellular Components
Multiphoton microscopy is especially useful when intrinsic fluorescence imaging is combined with other label-free imaging modalities, such as second-harmonic generation or coherent anti-Stokes Raman scattering, which may open new windows of...
BioPhotonics, September 2011
Multiplexed Sensing with QD-based FRET
For more than a decade, investigators have explored the potential of quantum dot (QD)-based Förster resonance energy transfer (FRET) for multiplexed diagnostics, noting the unique photophysical properties of QDs. This technique has not yet...
BioPhotonics, September 2011
Photoacoustic Microscopy Unlocks Secrets of Optical Absorption
Mainstream optical microscopy technologies cannot sensitively measure the optical absorption that provides essential physiological information such as the concentration and oxygen saturation of hemoglobin. In our research, we have implemented...
BioPhotonics, September 2011
Photon by Photon
Since the photomultiplier tube (PMT) was invented in the mid-1930s, it has remained the principal detector for experiments involving a small number of photons. However, its low photon detection efficiency and high sensitivity to magnetic fields...
BioPhotonics, September 2011
Fluorescence Illumination: New Metrics for Reproducibility and Quantitation
An exponential rise in quantitative fluorescence and live-cell work is driving a need for microscopists to understand, control and calibrate their instrumentation. For decades, there has been talk about a mechanism for measuring and calibrating true...
BioPhotonics, July 2011
Molecular Spectroscopy: Choosing the Right Instrument for the Job
As a field, molecular spectroscopy is as wide as the Mississippi River and as deep as the Mariana Trench. Alone or in conjunction with liquid and gas chromatography instruments, a good spectroscopy system can be used to explore DNA and RNA as well...
BioPhotonics, July 2011
Ophthalmic Lasers Expand from Surgery to Detection and Diagnosis
Lasers are an established surgical tool in an ophthalmologist’s armory against glaucoma, cataracts and macular degeneration, but developments in OCT will have doctors turning to lasers before the patient ever reaches the operating room. The...
BioPhotonics, July 2011
Optical Spectroscopy Gives a Sharp Gaze into Tissues
Various forms of spectroscopy are used to investigate the optical properties of tissue samples. Techniques such as diffused reflection and fluorescence spectroscopy are used to search for liver contaminants, to identify oils and lotions on skin, to...
BioPhotonics, July 2011
The (Never Ending?) Search for Higher-Resolution Microscopy
Can optical microscopy further shred the Abbe diffraction limit? Is it important to even try? Until about two decades ago, optical microscopists were stuck viewing their samples with a resolution no better than 200 nm. Now, well-funded labs have...
BioPhotonics, July 2011
Aesthetic Laser Market Is More Than Skin Deep
Sculpting, toning and smoothing: Aesthetic lasers can beautify us in ever more innovative ways. And with lasers operating at more wavelengths, configurations and options than ever, the number and range of treatments continue to expand, making the...
BioPhotonics, May 2011
Creating Cost-Effective Biomedical Filters
As the demand for high-precision biotech filters continues to rise, so does the need to drive down the cost of end products. While such filters have especially rigorous coating requirements , they are often overspecified for the intended...
BioPhotonics, May 2011
ECBO Promotes Advances in Biomedical Optics
The development and application of optical techniques and tools for biomedical imaging, diagnostics and therapy have been continuously expanding for several decades. These advances are based on multidisciplinary efforts requiring contributions from...
Photonics.com, May 2011
Laser Munich Brings Science and Industry Together
There is a lot of variety in photonics, and the Laser World of Photonics trade fair and World of Photonics Congress, to be held concurrently from May 23 to 26 at New Munich Trade Fair Centre, will cover the full spectrum of technologies. Industry...
Photonics.com, May 2011
Making Multiphoton Microscopy More Useful
Multiphoton microscopy techniques – including two-photon fluorescence and single-harmonic generation (SHG) – have blossomed over the more than 20 years since they first became widespread, but they continue to evolve, becoming more useful...
BioPhotonics, May 2011
New sCMOS vs. Current Microscopy Cameras
Since the launch in late 2010 of imaging cameras based on a new 5.5-megapixel scientific CMOS (sCMOS) sensor, there has been much speculation about whether sCMOS will become a technology replacement for interline CCD and electron-multiplying CCD...
BioPhotonics, May 2011
High-Power Diode Lasers for Laser Surgery
Laser-based surgery is staking an increasingly important claim in operating theaters across the world. More specifically, a class of devices called high-power diode lasers (HPDLs) provides numerous advantages over instrument-based surgery as well as...
BioPhotonics, April 2011
Protecting Food Resources through FTIR Spectroscopy
People have complicated relationships with food. Some eat too much, others too little. Many have no idea what they are eating; others are hyperaware and strive for the best dining experience possible. None of us wants to be misled about what we are...
BioPhotonics, April 2011
Time-Lapse Microscopy Captures Cells in the Act
Viewing biological processes as they happen provides a fascinating insight into cell behavior and can unveil vital clues about developmental milestones that have been puzzling scientists for some time. Time-lapse microscopy (TLM) involves...
BioPhotonics, April 2011
Tissue Imaging with Raman Spectroscopy and SERS
Recent years have seen an increase in the use of gold nanorods (GNRs) as contrast agents for in vivo optical imaging, with applications including near-infrared transmission imaging and photoacoustic tomography as well as surface-enhanced Raman...
BioPhotonics, April 2011
With lasers, surgery on the cutting edge
There’s more to laser surgery than meets the eye – meaning that there’s more to surgical lasers than vision correction. For instance, lasers are used to treat throat cancer, to remove excess prostate tissue and to remodel cells....
BioPhotonics, April 2011
Confocal microscopy gets smaller and faster – and branches out
George McNamara would like confocal microscopes to count. When McNamara, image core manager at the University of Miami’s Miller School of Medicine, says this, he isn’t talking about the importance of the four confocal instruments he...
BioPhotonics, March 2011
Fiber lasers are closing the technology gap
Ultrafast fiber lasers, found in countless research laboratories around the globe, are popular tools for physicists and biologists. Favored for being compact and reliable, fiber lasers also come at a fraction of the cost of comparable solid-state...
BioPhotonics, March 2011
Raman Allows Light-Therapy Monitoring
The goal of preventive medicine is to stop illness before it begins, or to discover and treat disease before it spreads and becomes serious. There are many approaches to preventive medicine today, including screening for high blood pressure and...
BioPhotonics, March 2011
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