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Pushing Sensitivity to the Brink: Selecting the Right Imaging Technology for Your Application
Knowledge of the most common sensor technologies and camera housing properties, along with their pros and cons in the context of low-light life sciences applications, will give users a better understanding of which camera parameters can increase a system’s output. With that information, the camera selection process can be illustrated through the design of a hyperspectral fluorescence-guided surgery device for brain tumor resection. Life sciences strongly benefit from the analysis of
BioPhotonics, December 2015
An Illuminated State of Mind
In a small laboratory with very little equipment, psychiatrist, neuroscientist and professor Karl Deisseroth began a high-risk project to render individual neurons photosensitive. Despite facing a great deal of skepticism from his peers, he remained...
BioPhotonics, December 2015
MPEF Microscopy Shines Best on Living Samples
MPEF microscopy, along with other nonlinear methods that include coherent anti-Stokes Raman scattering (CARS), stimulated Raman scattering (SRS) and second harmonic generation (SHG), is suitable for imaging whole cells — especially cells...
BioPhotonics, October 2015
In Vivo Neural Imaging — SLMs for Conducting 3D Holography inside Living Brains
The brain truly is a marvelous wonder of biology. With an extensive range of functions and dynamics that span many length scales and timescales, it is also marvelously complex. By better understanding our brains’ neural networks, researchers...
BioPhotonics, October 2015
OPSLs — Unique Benefits Driving Growth in the Life Sciences
In an OPSL, laser diodes are used to pump a thin semiconductor chip, thus enabling an incredible level of design flexibility in terms of power and wavelength scalability. The quantum well structure of the gain chip determines the OPSL’s output...
BioPhotonics, October 2015
Reduced to the Essentials — Portable Imaging Gets High-Tech
It takes some clever thinking to shrink many of the cumbersome biological imaging tools in use today to a size where they can be taken directly to the bedside. For some patients, point-of-care imaging is the only option. Portable imaging devices...
BioPhotonics, October 2015
Clinicians Demand More From Optical Devices
From the ubiquity of smartphones to an aging population, the modern world offers the optical sector a wealth of opportunities to innovate for the health care sector. Optical technologies have a long history of providing a rapid and noninvasive way...
Photonics.com, September 2015
Using QCLs for MIR-Based Spectral Imaging — Applications in Tissue Pathology
H. Sreedhar*1, V. Varma*2, A. Graham3, Z. Richards1, F. Gambacorata4, A. Bhatt1, P. Nguyen1, K. Meinke1, L. Nonn1, G. Guzman1, E. Fotheringham5, M. Weida5, D. Arnone5, B. Mohar5, J. Rowlette5 1 Department of Bioengineering, University of Illinois at...
BioPhotonics, September 2015
Multiwavelength TIRF Microscopy Enables Insight into Actin Filaments
Researchers at the University of California, San Francisco (UCSF) are combining multiple laser excitation wavelengths in total internal reflection fluorescence (TIRF) microscopy to investigate the binding dynamics of individual actin filaments. TIRF...
BioPhotonics, September 2015
Supercontinuum Sources Enhance UHR-OCT Methods
Ultrahigh-resolution optical coherence tomography (UHR-OCT) enables micron-scale, cross-sectional and 3D-imaging capabilities. Supercontinuum sources offer the necessary optical bandwidths, spatial coherence and optical power densities — all...
BioPhotonics, September 2015
Sterilization Effects on Optical Fibers for Clinical Use
Optical fibers have a broad range of uses in medical and clinical applications and can be adapted for many invasive and noninvasive procedures. However, any optical fiber to be used inside a human body first must be sterilized. This article reports...
BioPhotonics, September 2015
Lasers Make Their Marks on Skin and Tissue Procedures
Light-based aesthetic procedures are advancing from well-established spa-based procedures to trickier problems, such as resistant tattoo removal and treatments for wounds, scars and skin diseases. Laser hair removal was the number one laser-based...
BioPhotonics, August 2015
Optimizing Experimental Conditions for STED and g-STED Microscopy
The advent of superresolution microscopy has been groundbreaking in its ability to image biological samples, allowing researchers to study previously inaccessible details within cellular processes. New software provides a unique imaging optimization...
BioPhotonics, August 2015
SBF Imaging – Using SEM and LM to Create High-Resolution 3-D Reconstructions
In recent years, there have been considerable advancements in EM-based methods for 3-D reconstructions of large tissue volumes. In this article, we describe a new approach that combines in situ serial sectioning with a unique, multienergy...
BioPhotonics, August 2015
Laser World of Photonics
Laser Munich will focus on image processing, from products and systems to manufacturing and applications. Image processing and laser systems are ever-present in modern manufacturing. The 2015 Laser World of Photonics conference and trade fair,...
Photonics.com, June 2015
Single-Molecule Localization Microscopy with sCMOS Cameras
EMCCD-based cameras with high quantum efficiencies and low readout noise characteristics traditionally have been the preferred technology for SMLM superresolution imaging. However, with tailored localization algorithms, sCMOS cameras have become a...
BioPhotonics, June 2015
Spectroscopy Aids Disease Detection, Faces Obstacles
Spectroscopy advances are steadily reducing disease-detection time frames. These new methods also hone the accuracy of disease identification and provide for more timely treatment protocols. As with anything groundbreaking, though, roadblocks...
BioPhotonics, June 2015
Nanodiamonds Shine New Light on Bio Applications
Defect complexes in nanodiamond particles, which consist of vacancies trapped by nitrogen atoms, form different color centers depending on the nitrogen state present in the diamond. New research into these color centers indicates that nanodiamond...
BioPhotonics, June 2015
Femtosecond Pulses: Control Is Key to New Discoveries
From microscopy to micromanipulation, femtosecond pulses are broadening their reach throughout the photonics research world. To fully realize their potential, precise pulse control is critical. Ultrafast lasers have transformed the worlds of...
BioPhotonics, June 2015
Advances in Optical Filters Provide Rapid, On-the-Go Diagnosis
When it comes to diagnostics, one of the most crucial – and often overlooked – components is the optical filter. But as manufacturing processes improve, optical filters of exceptional quality are being created, which not only deliver...
BioPhotonics, April 2015
Comparing Micromanipulators for Electrophysiology
Several different technologies offer a range of performance options within the confines of an optical microscope for patch-clamp studies, intracellular recording and other demanding experiments in electrophysiology. Electrophysiology encompasses the...
BioPhotonics, April 2015
Focus-Tunable Lenses Enable 3-D Microscopy
Focus-tunable lenses speed up and enhance three-dimensional imaging capabilities in a diverse set of modern microscopy methods including confocal, two-photon and light-sheet microscopy. Microscopy beginners may be perplexed when they notice that...
BioPhotonics, April 2015
Lasers for Microscopy: Major Trends
Laser development for the microscopy market continues to be driven by key trends in applications, which currently include superresolution techniques, multiphoton applications in optogenetics and other areas of neuroscience, and even a shift in...
BioPhotonics, April 2015
Femtosecond Lasers Provide a Gentler Way to Micromachine
From the unbreakably hard to the vulnerably soft – from glass, metals and ceramics to plastics and biological tissue – femtosecond lasers are being increasingly adopted for micromachining a range of materials. Femtosecond lasers are...
Photonics.com, March 2015
Lasers, Optics Enhance Optogenetics Studies
A guide to suitable light sources and fiber optic components for optogenetics research, with a specific emphasis on rodent behavior. The use of lasers and optics for optogenetics is only in its infancy. In the next decade, we can expect researchers...
Photonics.com, March 2015
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