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Simplifying Flow Cytometry Through Panel Design

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No/low compensation panels reduce complexity of immunophenotyping assays while generating high-quality data from unique signals.

Sharon Sanderson, Bio-Rad Laboratories

Flow cytometry is an analytical technique that enables rapid, high-resolution analysis of diverse cell populations at high throughput. In fields such as immunology, oncology, and drug discovery, the ability to identify different cell types from a test sample suspended in fluid accelerates breakthroughs in understanding disease mechanisms and effective therapies. By using antibodies conjugated to fluorophores, flow cytometry facilitates the detection and quantification of cell surface, intracellular, and nuclear markers across diverse cell populations. Immunoglobulin, the most common...Read full article

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    Published: March 2025
    Glossary
    flow cytometry
    Flow cytometry is a powerful technique used in biology and medicine for the quantitative analysis of the physical and chemical characteristics of cells and particles suspended in a fluid. The method allows for the rapid measurement of multiple parameters simultaneously on a cell-by-cell basis. It is widely used in various fields, including immunology, microbiology, hematology, and cancer research. Here are the key components and features of flow cytometry: Sample preparation: Cells or...
    multiplexing
    The combination of two or more signals for transmission along a single wire, path or carrier. In most optical communication systems this is referred to as wavelength division multiplexing, in which the combination of different signals for transmission are imbedded in multiple wavelengths over a single optical channel. The optical channel is a fiber optic cable or any other standard optical waveguide.
    Featuresflow cytometrycompensation panelsfluorophoresantibodiesphenotypingmultiplexingnonspecific cell bindingspectral overlapBio-Rad LaboratoriesdyesLasersFilters

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