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Kinetic River Develops Autofluorescence Removal Technology

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Flow cytometry instrumentation company Kinetic River Corp. completed a small business innovation research (SBIR) project funded by the National Institutes of Health (NIH). The award funded the development of the company’s “Colorado” automated autofluorescence removal technology.

“Through a combination of hardware and algorithm development, we have been able to demonstrate the removal of the autofluorescence contribution from a population of exogenously stained eosinophils — a cell type with notoriously high autofluorescence signal,” said Giacomo Vacca, president of Kinetic River.

The completion of the Phase I program paves the way for further development and commercialization of a four-laser, 16-channel Colorado instrument with fully automated removal of the contribution from cellular autofluorescence, the goal of a Phase II SBIR proposal recently submitted to the NIH.
Lambda Research Optics, Inc. - Large Optics

Published: March 2021
Glossary
flow cytometry
Flow cytometry is a powerful technique used in biology and medicine for the quantitative analysis of the physical and chemical characteristics of cells and particles suspended in a fluid. The method allows for the rapid measurement of multiple parameters simultaneously on a cell-by-cell basis. It is widely used in various fields, including immunology, microbiology, hematology, and cancer research. Here are the key components and features of flow cytometry: Sample preparation: Cells or...
fluorescence
Fluorescence is a type of luminescence, which is the emission of light by a substance that has absorbed light or other electromagnetic radiation. Specifically, fluorescence involves the absorption of light at one wavelength and the subsequent re-emission of light at a longer wavelength. The emitted light occurs almost instantaneously and ceases when the excitation light source is removed. Key characteristics of fluorescence include: Excitation and emission wavelengths: Fluorescent materials...
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