About This Webinar
Multiscale Cleared Tissue Axially Swept Light-Sheet Microscopy (MCT-ASLM) addresses a core challenge in biological imaging: visualizing rare events or structures distributed across large, complex tissues. By combining centimeter-scale fields of view with targeted, high-resolution imaging at ~300 nm, this new microscopy platform enables researchers to examine entire specimens and seamlessly zoom in to investigate finer cellular or subcellular details.
A key component of MCT-ASLM is the navigate software, an intelligent image acquisition software that autonomously recognizes and interrogates areas of interest, streamlining data collection in thick, cleared tissue samples. This innovative approach significantly reduces operator workload while ensuring that critical yet rare events — such as sparse neuronal populations, metastatic lesions, or subtle developmental structures — are discovered and recorded.
In this presentation, Kevin Dean will highlight the successful application of MCT-ASLM across diverse model systems. By integrating automation, extensive volume coverage, and subcellular resolution, MCT-ASLM opens new avenues for comprehensive tissue analysis. The platform holds immense promise for accelerating discoveries in neuroscience, oncology, and developmental biology, offering new insights into the complexities of biological systems.
Who should attend
R&D Scientists, biomedical engineers, and manufacturers who work in life science imaging and instrument development. Those who work in imaging, microscopy, oncology, neuroscience, clinical research, cell biology, and biotechnology.
About the presenter
Kevin Dean is an interdisciplinary scientist who develops and applies advanced technologies to understand how tissue function arises from the organization and activity of proteins at subcellular scales. He earned his B.A. in chemistry from Willamette University in 2007 and his Ph.D. in biochemistry from the University of Colorado Boulder in 2013 under the mentorship of Amy Palmer. His expertise spans a range of fields, including thermodynamics, optical probes, nonlinear spectroscopy, microfluidics, advanced microscopy, cell biology, and computer vision.
Dean leads key efforts in two multisite Center Grants: the NCI Cellular Cancer Biology Imaging Research Center, where he is developing an autonomous multiscale microscope to identify and isolate single metastatic cells in cleared tissues, and the NIGMS Biomedical Technology Development and Dissemination Center, where he advances and disseminates cutting-edge light-sheet microscopy techniques. His contributions to microscopy — such as axially swept light-sheet, field synthesis, and oblique plane microscopy — enable live-cell imaging and reveal new insights into cellular signal transduction through multiplexed biosensors and sophisticated time-series analysis.
Outside of the lab, Dean enjoys spending time with his two young children, ages four and one and a half.