Video tutorial about diSPIM alignment with Jon Daniels.
- Setting the Limit Magnets on the Microscope Stage
- Overview of the Fine Alignment
- Aligning the Beams and the Objectives
- Aligning the Beams
- Adjusting the Dichroics
- Adjusting the Cameras
- Re-Adjusting Everything
- Adjusting the Collimators
- Adjusting the Scanners
- Going Live with the Fluorescent Bead Solution
- Spiraling In Repeat All Adjustments Again
- Lowering the SPIM Head to the Coverslip and Checking that the SPIM Head is Rotated Correctly
- Piezo and Scanner Cross Calibration
- Focusing and Aligning the Bottom Objective