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Photonics Handbook
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Superresolution Microscopy: An Imaging Revolution
Marie Freebody, Contributing Editor
Superresolution optical microscopy, for which the Nobel Prize was awarded to Eric Betzig, Stefan Hell, and William Moerner in 2014, has been one of the most momentous developments in the life...
Fluorescence Lifetime Imaging: Choosing the Best Approach
GERHARD HOLST, Excelitas PCO GmbH
The term fluorescence is often applied as a synonym for photoluminescence, although luminescence actually covers fluorescence and phosphorescence. Both of these terms describe the process of...
Microscopy Illumination: Considering Nonlaser Light Sources
BARBARA FOSTER, THE MICROSCOPY & IMAGING PLACE INC.
As recently as five years ago, shopping for a microscope light source was fairly straightforward. For routine applications, it was tungsten halogen, typically 100 W, while for fluorescence, it was an...
Lasers for Microscopy: Major Trends
Marco Arrigoni, Nigel Gallaher, Darryl McCoy, Volker Pfeufer, Matthias Schulze, and Daniel Callen, Coherent Inc.
Laser development for the microscopy market continues to be driven by key trends in applications, which currently include superresolution techniques, multiphoton applications in optogenetics and...
Detectors: CCDs for Life-Science Applications
Butch Moomaw, Hamamatsu Corporation, Systems Div.
Since their invention in the late 1960s, charge-coupled devices, also called CCDs, have found widespread use in imaging applications. Electronic cameras based on CCD technology are used in...
Nd:YAG Lasers: Standing the Test of Time
Quantel USA
The ubiquitous Nd:YAG laser has played many roles over the years. For the military, it has provided rangefinding and target designation capabilities. When used with nonlinear optics or as a pump...
Hyperspectral Imaging Spectroscopy: A Look at Real-Life Applications
Dr. John R. Gilchrist, Clyde HSI; Timo Hyvärinen, Spectral Imaging Ltd.
Hyperspectral imaging spectroscopy has developed dramatically from a large, complex, remote-sensing satellite- or aircraft-based system into a rugged, compact, economically priced imaging and...
Digital Still Cameras: The Changing Face of Imaging
Morio Onoe, Professor Emeritus, University of Tokyo
The digital camera represents an integration of optics, mechanics and electronics consisting of three layers (Figures 1a, b and c). The top and the middle layers are printed circuit boards (PCBs),...
Tunable Lasers: Generating Wavelengths from the UV Through the IR
Ian Read, MKS/Spectra-Physics
Applications facilitated by tunable lasers fall into two categories: situations in which one or more discrete wavelengths are not available from any single- or multiline fixed-wavelength laser, or...
Spectroscopy: The Tools of the Trade
Dr. John R. Gilchrist, Clyde HSI
All optical spectrometry techniques rely on the measurement of radiant power. The configuration of the instrument varies based on the measurement technique: absorption, emission, luminescence, or...
Particle Image Velocimetry: Basics, Developments and Techniques
M. Kelnberger, InnoLas GmbH; G. Schwitzgebel, Universität Mainz
Particle image velocimetry (PIV) is an experimental tool in fluid mechanics and aerodynamics. The basic principle involves photographic recording of the motion of microscopic particles that follow...
Colorimetry: How to Measure Color Differences
Konica Minolta Sensing Americas Inc.
The use and importance of colorimetry has grown in unison with the increase of global manufacturing and processing. When plastic automotive trim produced on one continent must match a painted metal...
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