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Meadowlark Optics - Wave Plates 6/24 LB 2024

Spectral Confocal Microscopy Aids FRET Studies

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Förster resonance energy transfer (FRET) provides a powerful tool for revealing interactions between molecules on a subcellular level.

Jeffrey M. Larson and Dr. Jennifer L. Peters, Nikon Instruments Inc.

FRET is an energy transfer between two fluorescent molecules that occurs without the exchange of a photon. It occurs when a fluorescent molecule, usually called the donor probe, is excited by the absorption of a photon. If a fluorescent probe that can be excited by the energy held in the excited state of the donor molecule is very near, resonance energy transfer can occur between the molecules. This second molecule, called the acceptor probe, usually decays to the ground state by the emission of a photon of a longer wavelength than that expected from the donor probe.1 The FRET signal is...Read full article

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    Published: July 2006
    Basic ScienceBiophotonicsFeaturesMicroscopySensors & Detectors

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