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Smart Probe Captures Biomarker Sequence

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A fluorescently labeled nucleic acid instrument with a stem-loop structure helps to identify a specific cancer microRNA sequence.

SULAYMAN A. OLADEPO, KING FAHD UNIVERSITY OF PETROLEUM AND MINERALS

A smart probe is a hairpin oligonucleotide, essentially a string of nucleic acids, that is labeled with a fluorophore on one end and tethered with successive guanosine residues on the other end1. Extrinsic dye labels with suitable absorption are typically used for fluorescence quenching, which signals a reaction. But recent research has shown that guanosine moieties can provide effective fluorescence quenching for common fluorescent dyes due to guanosine’s low oxidation potential2. Thus, guanosine is used as a quencher in smart probes, which can detect biomarkers of cancer and other...Read full article

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    Published: April 2021
    Glossary
    microrna
    MicroRNAs (miRNAs) are a class of small, single-stranded RNA molecules, typically consisting of about 19 to 24 nucleotides, that play important roles in the regulation of gene expression in cells. They are involved in post-transcriptional regulation, meaning they influence the translation of messenger RNA (mRNA) into proteins after the mRNA has been transcribed from DNA. The process by which miRNAs regulate gene expression involves several steps: Transcription: miRNA genes are...
    fluorophore
    A fluorophore is a molecule or a portion of a molecule that has the ability to emit light upon excitation by an external energy source, such as ultraviolet or visible light. The process by which a fluorophore absorbs and then re-emits light is known as fluorescence. Fluorophores are widely used in various scientific and technological applications, including fluorescence microscopy, flow cytometry, medical imaging, and molecular biology. Key features of fluorophores include: Excitation and...
    smart probemicroRNAfluorophorequencherguanosineperfect target sequencemismatch target sequenceKing Fahd University of Petroleum and MineralsFeaturesBiophotonicsTest & MeasurementImaging

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