Nikon, Agilent to Make Laser-based Biomed Imagers

MELVILLE, N.Y., Jan. 19, 2011 — Advanced optical instruments provider Nikon Instruments Inc. and Agilent Technologies, a measurement company, announced an agreement for distribution in the Americas of Agilent’s MLC400 monolithic laser combiner systems, which will be integrated into laser systems designed for Nikon’s Ti-E PFS “Perfect Focus’ inverted research microscope.

The integration of the systems will allow researchers to maximize productivity and minimize system down time. Incorporated into the Ti-E PFS system—capable of multi-day live-cell imaging experiments—and under the control of NIS-Elements Advanced Research, the integration can be run at camera frame rate or faster.

“The combination of Nikon’s superior imaging optics with Agilent’s expertise in precision optics and laser systems makes this a natural synergy,” said Stephen Ross, general manager of product and marketing for Nikon. “We believe this agreement will provide our customers with both the imaging and laser capabilities they require for high-end research and experimentation.”

The two companies will initially begin integration of the systems for total internal reflection fluorescence, photobleaching/photoactivation and field scanning confocal systems.

For more information, visit: www.nikoninstruments.com

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Published: January 2011

Glossary

frame rate: Frame rate refers to the frequency at which consecutive images, or frames, are displayed in a video sequence. It is typically measured in frames per second (fps) and determines the smoothness and perceived motion of the video. In digital video, each frame consists of a snapshot of the scene at a particular moment in time. When these frames are played in rapid succession, the illusion of motion is created. The frame rate dictates how many frames are displayed per second, thus affecting the...
microscope: An instrument consisting essentially of a tube 160 mm long, with an objective lens at the distant end and an eyepiece at the near end. The objective forms a real aerial image of the object in the focal plane of the eyepiece where it is observed by the eye. The overall magnifying power is equal to the linear magnification of the objective multiplied by the magnifying power of the eyepiece. The eyepiece can be replaced by a film to photograph the primary image, or a positive or negative relay...
photobleaching: Photobleaching is a phenomenon in which the fluorescence of a fluorophore (a fluorescent molecule or dye) is permanently reduced or eliminated upon prolonged exposure to light. This process occurs due to the photochemical destruction or alteration of the fluorophore molecules, rendering them non-fluorescent. Key points about photobleaching: Mechanism: Photobleaching is typically a result of chemical reactions induced by the absorbed photons. The excessive light exposure causes the...
total internal reflection fluorescence: Total internal reflection fluorescence (TIRF) microscopy is an advanced fluorescence microscopy technique that leverages the principle of total internal reflection to selectively illuminate and image a thin section of a specimen near the interface of two media, typically a glass coverslip and the sample medium. TIRF microscopy allows for high-resolution imaging of fluorescently labeled structures near the cell membrane or other interfaces, offering improved signal-to-noise ratios and reduced...

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