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Microscopy News
Imaging method measures width of brain’s extracellular space
Jun 1, 2006 — Drugs and drug carriers targeting the brain can range from less than one to several hundred nanometers in size and must travel through the brain tissue before being directed to target sites. Knowing the structure and width of the brain tissue’s extracellular space could help scientists design effective drug treatment strategies. Previous electron microscopy experiments have suggested that the brain’s extracellular space is between 10 and 20 nm wide — obviously too small...
Loading the protein researcher’s imaging toolbox with fluorophores
Jun 1, 2006 — Over the past several years, an increasing number of fluorescent probes — from both natural and laboratory sources — have made possible noninvasive imaging of live cells and even whole organisms. And, according to Roger Y. Tsien and...
Microcomputed tomography aids tissue engineering scaffold design
Jun 1, 2006 — In tissue engineering, where the goal is to create artificial structures that mimic living organs such as skin or bone, researchers frequently use a scaffold-based approach. Scaffolds, which help support and bind the cellular elements of the tissue...
Microscope Takes Deep-UV and Visible Picosecond Fluorescence Lifetime Measurements
Jun 1, 2006 — Time-resolved microscopy is the ultimate tool for investigating dynamic events in cells and subcellular structures. However, it previously was limited when used for applications that required wavelengths longer than 370 nm because of microscope...
Multispectral FLIM Enhances FRET Autofluorescence Imaging
Jun 1, 2006 — er the past decade, confocal and multiphoton laser scanning microscopy have become standard for biomedical imaging on the cellular level. Because these techniques either suppress or don’t generate out-of-focus light, they produce clear images...
New physical model reveals information on causes of cellular damage
Jun 1, 2006 — A variety of neurodegenerative disorders — including Alzheimer’s, Parkinson’s and Huntington’s diseases — have been linked to oxidative stress, in which free radicals chemically attack cells and parts of cells....
On the Path Toward More Useful Fluorophores
Jun 1, 2006 — Where would modern biomedical research be today without fluorescent probes? Some would argue that it would be blindly groping down a dark alley, with no efficient means of discovering the finely honed interplay of proteins and other substances in...
One laser does the work of three
Jun 1, 2006 — When it comes to laser sources, three are definitely a crowd. That is particularly true when trying to align multiple beams to illuminate the same confocal volume. So researchers at the National University of Singapore used just one beam and...
Tracking the infection of flies
Jun 1, 2006 — Although it is known that Wolbachia — a bacterial genus present in 20 to 80 percent of all insects — is transmitted from female hosts to their offspring through their germ line, it has only been suspected that infectious transmission...
Twin-beam optical trapping spins cells
Jun 1, 2006 — Detecting the causes of cancer at its earliest stages would be of great help in fighting the disease. However, the sooner one tries to see the beginnings of tumor development, the less change is visible in a cell’s components and in their...
A ‘dark’ fluorescent protein could aid FRET experiments
May 1, 2006 — Microscopy using Förster resonance energy transfer is a potent method for imaging protein conformations and interactions. However, the technique has two conflicting requirements: the necessary spectral overlap between the donor molecule’s...
Adding metal film to total internal reflection microscopy enhances images
May 1, 2006 — Anyone who uses fluorescence detection of single molecules to investigate protein structures and dynamics within cells eventually faces the problem of isolating individual molecules from areas with high concentrations of proteins, such as muscle...
Aperio Technologies Inc.
May 1, 2006 — Aperio Technologies Inc. of Vista, Calif., has secured an exclusive reseller license for Zoomify Inc.’s third-party Web viewing software. The program enables Internet viewing of industry-standard digital slide files from any standard Web...
Blue light changes a fluorophore from green to red
May 1, 2006 — During the past several years, researchers have developed photoactivatable fluorescent proteins that are similar to GFP but that change color when introduced to UV-to-violet wavelengths. Unfortunately, UV radiation can be damaging to some of the...
Building protein microstructures with less expensive lasers
May 1, 2006 — The emission from a femtosecond Ti:sapphire laser can be used to create miniature structures made of protein that fence in a living bacterium. Fabricating structures in this way could someday be used to engineer neuronal networks for prosthetics or...
Creating Clarity: Adaptive Optics for Bioimaging
May 1, 2006 — Biological imaging instruments often have resolution limitations that restrict the ability of researchers and clinicians to detect critical detail. One reason is that, as light passes through tissue to reach the object of interest — a cell,...
Diamond biosensors are forever
May 1, 2006 — To make effective biosensors, the detection molecules must be attached to a microelectronics-compatible surface in such a way that it is highly stable for long periods. Combinations of biomolecules and commonly used substrate materials such as...
Fluorescence lifetime shows temperatures of flowing liquids
May 1, 2006 — Many biological analytical processes would benefit from miniaturization, but some —such as DNA amplification through polymerase chain reaction (PCR) — depend on homogeneous temperature gradients to optimize the chemical or molecular...
Focusing on the Experiment
May 1, 2006 — For Alexey Khodjakov, a researcher at the New York State Department of Health’s Wadsworth Center in Albany, it wasn’t a question of if but when he would lose focus. Following cells as each divided into many, he and colleagues in his lab...
Hardware, positioners
May 1, 2006 — Melles Griot has added to its online catalog a full line of optomechanical hardware, including optical rail and mounting systems, controllers for nanometric positioning, high-resolution positioning stages with manual or motorized actuators, and...
Imaging is a key to progress in stem cell research
May 1, 2006 — An important aspect of stem cell research is the function of the cells that support them and enable them to function properly. These systems of supportive cells — called niches — sequester stem cells from apoptotic and differentiation...
Imaging methods
May 1, 2006 — Part of the “Methods Express” series, Cell Imaging covers key techniques that can be used in laboratories with access to cell imaging equipment. Although the 350-page book focuses on live-cell imaging and light microscopy applications,...
Laser-activated bubbles mitigate toil and troubles
May 1, 2006 — A number of biomedical applications take advantage of laser light interactions with tissue that are accompanied by absorption of light by tissue. As the absorbed energy converts into heat, it produces transient nonuniform thermal fields in cells,...
Miniature 2-D flow cytometer
May 1, 2006 — Conventional flow cytometers are large and expensive and usually provide one-dimensional data, but two-dimensional data is needed for detecting subtle differences in the morphology of cells. Researchers from the University of Alberta in Edmonton,...
Miniature lenses for tiny microarray spots
May 1, 2006 — Although microarrays may seem small, they’re too big, at least for portability. Miniaturizing them further is difficult because they consist of many sensing spots. The individual spots are small, but most arrays manufactured today are...
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May/Jun 2024
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As Biosensors Shrink, Their Potential Applications Grow
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