Camera Selection for Low-Light Imaging
Fluorescence microscopy is a broadly used term, with applications ranging from basic life science, such as time-lapse cell viability assays, to sophisticated techniques in which a very few photons or single molecules are detected and localized by specific high-end hardware and software. It further extends to even more sensitive biochemistry detection methods that leverage, for example, chemiluminescence and bioluminescence. Low-light imaging technology is instrumental to all of these microscopy applications, and often finds use in macroscopic optical systems. Although fluorescence microscopy technology is quite mature, camera selection for specific applications remains challenging. It can be complex to design a cost-effective optical imaging system that is optimized for a particular task, and end users can benefit from an overview of the major technical aspects that should be considered when choosing imaging components.
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