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Biophotonics Monthly: FRET Microscopy Brings Us into a World of Molecular Interactions (5/22/2013)

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FRET Microscopy Brings Us into a World of Molecular Interactions FRET Microscopy Brings Us into a World of Molecular Interactions
Förster resonance energy transfer microscopy is not bound by the same restrictions as conventional light microscopy – it goes beyond the diffraction limit, allowing molecular interactions to be visualized with greater resolution than ever. The resolution of conventional light microscopes is limited by the wavelength of the light used to illuminate the specimen. FRET microscopy, on the other hand, detects the consequence of the direct transfer of excitation energy from one fluorophore to another nearby molecule. This enables us to see interactions between molecules or conformational changes within molecules at distances of less than 10 nm, far below the typical diffraction limit of light microscopes at around 200 nm.
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Eyes Wired to Spinal Cord Instead of Brain Can Still See
Transplanted eyes located far outside the head in a vertebrate animal model can see even without a direct neural connection to the brain, researchers have shown for the first time. The connections were tested using fluorescence microscopy, an LED light setup and camera-based motion-tracking technology.
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Q&A: Market Growing Slowly for PDT
Photodynamic therapy holds promise for cancer treatment and more, but it has yet to make a strong move into the clinic. Photodynamic therapy is not exactly new, but it’s still working on getting its foot in the door – the door of the clinic, that is. Still mainly in the research stages, PDT technologies and techniques have applications in treatment of cancer and other diseases. But the market hasn’t exploded just yet, insiders say.
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In this edition of the industry's premier weekly newscast: The wheels come off the Kepler space telescope, nanoantennas improve infrared sensing, and Managing Editor Laura Marshall shares her final impressions of Laser World of Photonics 2013. Co-hosted by Photonics Media Senior Editor Melinda Rose.
Images Reveal Cell Organization, Behavior and More
Scientists from various disciplines came together to discuss the latest imaging techniques and innovations at the recent American Association for the Advancement of Science (AAAS) annual meeting. Interdisciplinary collaboration is vital to biophotonics, especially in the area of imaging. A special symposium called “Innovations in Imaging: Seeing is Believing” brought physicists and cell biologists together to discuss collaboration and innovation in microscopic imaging.
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Laser-Based Breast Cancer Test Faces Human Trials
A laser diagnostic test that could lead to an instant diagnosis of breast cancer at the time of a mammogram will for the first time be evaluated using excised human breast tissue and lymph nodes. The method, known as spatially offset Raman spectroscopy (SORS), uses a laser to pinpoint objects deep beneath the skin – without an incision.
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Computational Microscopy, Sensing and Diagnostics for Telemedicine and Global Health Applications
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Photonics Media will host Dr. Aydogan Ozcan, Associate Professor, Bio- and Nano-Photonics Laboratory, Electrical Engineering and Bioengineering Departments, UCLA. Dr. Ozcan will introduce new imaging and detection architectures that compensate digitally for the lack of complex optical components available in cell phones by using novel theories and algorithms to address the immediate needs of telemedicine for global health problems.
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