< More MicroscopySubscribe to our E-NewslettersMicroscopy Tech Pulse (3/27/2018)
Microscopy Tech Pulse
Microscopy Tech Pulse is a special edition newsletter from Photonics Media and Mad City Labs covering key developments in microscopy technology.
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March 2018
Microscopy Tech Pulse is a special edition newsletter from Photonics Media and Mad City Labs Inc. covering key developments in microscopy technology. Manage your Photonics Media membership at Photonics.com/subscribe.
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Histology Breaks a Speed Barrier
Today’s optical instruments are outpacing human bandwidth and forcing their human designers and end users to operate more like orchestrators of complex, interlocked components. An integrated, automated microtome and microscopy platform sections and images tissue samples thousands of times faster than a human.
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Mad City Labs Inc.
Fluorescence Microscopy Technique Provides New Views of Biological Processes
Introduction to the technique of MicroMirror TIRF (total internal reflection fluorescence) microscopy. The MicroMirror TIRF microscopy technique offers significant signal-to-noise ratio improvements compared to dichroic based TIRF microscopy when using multiple wavelength light sources. Short overview of the types of biological processes that can be studied via this method. Mad City Labs is the only commercial provider of a MicroMirror TIRF microscope.
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Microscope Provides Precise 3D Imaging of Thick Mammalian Cells
TILT3D — tilted light-sheet microscopy with 3D point spread functions (PSFs) — combines a tilted light-sheet illumination technique with long axial range PSFs for low-background, 3D super-localization of single molecules and for 3D superresolution imaging in thick cells. The microscope produces 3D nanoscale images of mammalian cells in their entirety.
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MUSE: Simple Slide-Free Microscopy
A new technique skips traditional histology steps to speed up diagnosis and allow for views that are not possible with conventional sectioning. Even in this era of molecular medicine, conventional light microscopy remains the principal contributor to cancer diagnostics and also serves to guide surgical procedures, monitor disease activity and follow therapy responses over time.
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Optical Tool Uses Fluorescence to Detect Metabolic Changes
An optical tool that can read metabolism at subcellular resolution could be used to identify specific metabolic signatures indicative of disease. The method detects functional and structural metabolic biomarkers noninvasively using endogenous two-photon excited fluorescence (TPEF) from two coenzymes that are involved in a large number of metabolic pathways in every cell.
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Researchers at the University of Central Florida are using single-molecule pull-down (SiMPull) assays to understand and diagnose Parkinson’s disease. The team used single-molecule total internal reflection fluorescence (TIRF) microscopy to visualize single proteins captured by fluorescently labeled antibody and in vivo crosslinker to preserve native states of biological samples including cultured cells and postmortem brain tissues.
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During surgical procedures, it is imperative to rapidly distinguish between cancer and surrounding healthy tissue and to determine where and how much tissue to cut. Several clinical applications have demonstrated that optical molecular imaging can serve as a powerful intraoperative tool for guiding surgeons performing surgical interventions, including robotic procedures.
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