Single-Objective Light Sheet

• Company: Applied Scientific Instrumentation Inc.
• Type: Optical
• Applications: Medical/Clinical Science, Life Science
• Magnification: 67X
• Spectral Range: VIS
• Field of view: ≤350µm
• Measured resolution in X: ≅450nm
• Measured resolution in Y: ≅350nm
• Measured resolution in Z: ≅1.1µm

The single sample-facing objective is used for both light sheet illumination and detection. The illumination light sheet and detection plane are scanned together at the sample using a galvo, and the stationary descanned image is captured by a camera. This scheme allows volumetric imaging at camera-limited frame rates with negligible bleaching and excellent optical sectioning.