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Photonics Suppliers Fluorescence Microscopes

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Edinburgh Instruments Ltd.
A Techcomp Instruments Europe Co.

1 Bain Square
Kirkton Campus
Livingston EH54 7DQ
United Kingdom

Phone: +44 1506 425300

Fax: +44 1506 425320

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Multiphoton Imaging of Mouse Intestine

Wednesday, October 19, 2022
Two-photon excited fluorescence (2PEF) and second harmonic generation (SHG) are complementary multiphoton imaging techniques for studying biological samples. Both utilise femtosecond pulsed infrared excitation light to generate shorter wavelength light to image samples but operate via different physical processes. Both take advantage of the lower scattering and absorption of infrared light to enable deep tissue imaging. In this note, a Confocal Raman Microscope is used to image a tissue section of mouse intestine using 2PEF and SHG microscopy.

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