diSPIM Alignment Video - Applied Scientific Instrumentation

Video tutorial about diSPIM alignment with Jon Daniels.

Setting the Limit Magnets on the Microscope Stage
Overview of the Fine Alignment
Aligning the Beams and the Objectives
Aligning the Beams
Adjusting the Dichroics
Adjusting the Cameras
Re-Adjusting Everything
Adjusting the Collimators
Adjusting the Scanners
Going Live with the Fluorescent Bead Solution
Spiraling In Repeat All Adjustments Again
Lowering the SPIM Head to the Coverslip and Checking that the SPIM Head is Rotated Correctly
Piezo and Scanner Cross Calibration
Focusing and Aligning the Bottom Objective

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