Gold Nanoparticle-Based Laser System Modeled for Cell Transfection

An optically induced method has been modeled for an efficient and cell-preserving transfection — the delivery of DNA or RNA into eukaryotic cells — in high-throughput screening.

Selective manipulation of cells with fluorescent 10 kDa dextrans: The result of targeted laser irradiation using a laser mask can be seen under a microscope. Scale bars 10 mm. Courtesy of LZH.

The functional model for a gold nanoparticle-based laser transfection was reported by the Laser Zentrum Hannover e.V. (LZH) and its partner, working within a project of the Industrial Joint Research (IFG) of German’s Federal Ministry for Economic Affairs and Energy.

The transfection method is characterized by molecules entering the cells through an optically induced process. By attaching the gold nanoparticles to the cells, a photothermal effect would be achieved under laser irradiation, enabling an efficient transfer of molecules into the cells.

Illustration of the functional model for high-throughput screening. Courtesy of LZH.

LZH said the use of an optical mechanism makes the method more independent from the cell type and molecules used, and, within larger samples, the transfection can be carried out both more precisely and cell specifically.

The project has been successfully completed by presenting a functional model, with research ongoing toward the goal of commercialization. LZH is looking for partners from the screening area to build a prototype in the follow-up project.