In the life sciences category, Gopi Shah of the Max Planck Institute of Molecular Cell Biology and Genetics in Dresden, Germany, used a home-built light-sheet microscope setup with two Andor Zyla scientific CMOS cameras to capture video of a developing zebrafish embryo where each cell nucleus was labelled with green fluorescent protein. Two opposite views of the sample were fused post-acquisition to reconstruct the entire embryo in 3D.
Mátyás Molnár of Uppsala University in Sweden won the Imaris category using a light-sheet microscope in three channels with 405-, 488- and 561-nm laser excitation. The 3D dataset was converted into a maximum intensity projection image using Bitplane’s Imaris 3D/4D visualization and analysis software.
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