Lasers Align Molecules

Scientists have eliminated the need to crystallize human proteins for synchrotron x-ray diffraction by using lasers to align large groups of the molecules. With approximately one million human proteins incapable of being crystallized, the technique could revolutionize human protein imaging.

Scientists at Argonne National Laboratory devised the laser technique because of the difficulties protein crystallographers face when trying to crystallize molecules for drug-interaction research.

"Strong laser fields can be used to control the behavior of atoms and molecules," Argonne Distinguished Fellow Linda Young said. "Using x-rays, we can investigate their properties in a totally new way."

Crystallization allows scientists to create a periodic structure that will strongly diffract in specific directions when bombarded with x-rays. From the resulting diffraction pattern, a real-space image can be reconstructed.

However, without crystallization, when x-rays collide with multiple, randomly oriented molecules, they diffract in different directions, making it impossible to create a composite diffraction image, Argonne physicist Robin Santra said.

Some molecules, such as many involved with drug interaction, cannot be crystallized and imaging would require numerous samples to bombard in order to get a full composite picture. Young's laser technique allows for millions of molecules suspended in a gaseous state to be aligned so that when bombarded with x-rays, they all diffract in the same way. The resulting images are at atomic level resolution and do not require crystallization.

"Understanding the structure of the approximately one million human proteins that cannot be crystallized is perhaps the most important challenge facing structural biology," Young said. "A method for structure determination at atomic resolution without the need to crystallize would be revolutionary."

Young and her team have successfully aligned molecules using a laser, probed the aligned ensemble with x-rays and shown theoretically that the technique could be used for x-ray imaging, but they require a proposed upgrade to the Advanced Photon Source facility at Argonne before x-ray diffraction can be done experimentally, they said.

Funding for the research was provided by the DoE, Office of Science, and Office of Basic Energy Sciences. A paper on the work appeared in the March 8 issue of the journal Applied Physics Letters.

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