Sensor Tracks Insulin Level

A sensor built from multiwalled carbon nanotubes can rapidly and continuously measure tiny amounts of insulin in a microfluidic device. Used in conjunction with a new procedure for treating Type 1 diabetes through liver cell transplant, the device could help insulin-dependent diabetics go years between shots.

The new insulin detection method also represents a major step toward developing the ability to assess the health of the body's insulin-producing cells in real time, its creators said.

Close-up of the new sensor built at Vanderbilt University using carbon nanotubes. The sensor can measure the concentration of insulin produced by Islets of Langerhans cells at the same time as it measures other compounds that allow researchers to assess cells' health. (Photo courtesy Cliffel Laboratory)

A team of Vanderbilt University researchers headed by associate professor of chemistry David Cliffel developed the method by creating a new electrode for a microfluidic device called a microphysiometer, which assesses the condition of living cells by placing them in liquid nutrient, confining them in a very small chamber and then measuring variations in their metabolism. The volume of the chamber is only three microliters (µL) -- about 20 times smaller than an ordinary raindrop -- allowing the electrode to detect the minute amounts of insulin released by special pancreatic cells called Islets of Langerhans.

The electrode is built from multiwalled carbon nanotubes, which are like several flat sheets of carbon atoms stacked and rolled into very small tubes. The nanotubes, provided by William Hofmeister at the University of Tennessee Space Institute, are electrically conductive. The concentration of insulin in the chamber can be directly related to the current at the electrode and the nanotubes operate reliably at pH levels characteristic of living cells.

Current detection methods measure insulin production at intervals by periodically collecting small samples and measuring their insulin levels. The new sensor detects insulin levels continuously by measuring the transfer of electrons produced when insulin molecules oxidize in the presence of glucose. When the cells produce more insulin molecules, the current in the sensor increases and vice versa, allowing the researchers to monitor insulin concentrations in real time. It is similar to a device developed by another group of researchers that operated at acidity levels well beyond those where living cells can function.

Vanderbilt University research assistant professor Madalina Ciobanu, left, and graduate student Rachel Snider working with assistant chemistry professor David Cliffel in the laboratory. (Photo courtesy John Russell)

Previous tests had shown that nanotube electrodes are more sensitive at measuring insulin than conventional methods. However, the researchers had to overcome a major obstacle to adapt them to work in the microphysiometer.

In the small chamber, they found that the fluid moves across the electrode surface rather than pushing against it. These microcurrents tended to sweep the nanotubes aside rather than pinning them to the electrode surface where their electrical activity can be measured. The researchers solved this problem by mixing the nanotubes with a chemical called dihydropyran, a small molecule that forms chemical bonds that stabilize the nanotube film and stick it to the electrode surface.

“One of the key advances of this project was finding how to keep nanotubes active on the surface without being washed away by microfluidic flows,” Cliffel said.

Now that the microphysiometer has demonstrated the ability to rapidly detect the small quantities of insulin produced by individual cells, the researchers hope to use it to determine the health of the islet cells used in the new procedure for treating insulin-dependent diabetes -- liver cell transplantation.

Researchers at the University of Alberta in Canada have shown that islet cells can be transplanted into a Type I diabetic and can greatly relieve insulin dependence for several years. Unfortunately these transplants require large doses of immunosuppressive drugs, and scientists don’t yet know how these drugs affect the health of the islet cells.

Changing a debubbler membrane, which is part of the maintenance of the electrode. (Photo courtesy John Russell)

One of the next steps is to use the microphysiometer to measure insulin, lactate and oxygen levels simultaneously. This will allow researchers to study how the islet cells react to the drugs and help identify the best way to deal with transplant rejection. It will also allow them to verify the health of the islets cells before they are transplanted into patients.

The research, reported in the Feb. 18 issue of the journal Analytica Chimica Acta, was funded in part by the Vanderbilt Institute of Integrative Biosystems Research and Education and by a pilot project grant from Vanderbilt Diabetes Research and Training Center supported by the National Institutes of Health. It was performed with islet cells isolated from mice at the Vanderbilt Diabetes Research and Training Center.

Other authors of the study include graduate student Rachel M. Snider, research assistant professor Madalina Ciobanu, and former undergraduate researcher Amy E. Rue.

For more information, visit: www.vanderbilt.edu